Enhanced expression of hepatitis B surface antigen by sodium butyrate in PLC/PRF/5 human hepatoma cells

  • Authors:
    • F Mawatari
    • K Nakata
    • T Ueki
    • T Tsutsumi
    • A Ido
    • K Nakao
    • Y Kato
    • S Nagataki
  • View Affiliations

  • Published online on: August 1, 1996     https://doi.org/10.3892/ijo.9.2.285
  • Pages: 285-289
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Abstract

The effect of butyric acid, a natural fermentation product of colonic bacterial flora, on hepatitis B surface antigen (HBsAg) expression was investigated in HBsAg-positive PLC/PRF/5 human hepatoma cells. By Northern blot analysis, the levels of HBsAg mRNA increased dose-dependently using sodium butyrate (0-2 mmol/l). In transient chloramphenicol acetyltransferase plasmid transfection experiments, the HBsAg-preS2 promoter activity as well as the HBV enhancer 1 activity was stimulated by sodium butyrate, whereas the HBsAg-preS1 promoter activity was not. These results indicate that butyric acid functions as a physiological regulator of HBsAg expression through the portal blood flow and possibly contributes to increased expression ratio of preS2/S to preS1 polypeptides recognized in persistant HBV infection.

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August 1996
Volume 9 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mawatari F, Nakata K, Ueki T, Tsutsumi T, Ido A, Nakao K, Kato Y and Nagataki S: Enhanced expression of hepatitis B surface antigen by sodium butyrate in PLC/PRF/5 human hepatoma cells. Int J Oncol 9: 285-289, 1996
APA
Mawatari, F., Nakata, K., Ueki, T., Tsutsumi, T., Ido, A., Nakao, K. ... Nagataki, S. (1996). Enhanced expression of hepatitis B surface antigen by sodium butyrate in PLC/PRF/5 human hepatoma cells. International Journal of Oncology, 9, 285-289. https://doi.org/10.3892/ijo.9.2.285
MLA
Mawatari, F., Nakata, K., Ueki, T., Tsutsumi, T., Ido, A., Nakao, K., Kato, Y., Nagataki, S."Enhanced expression of hepatitis B surface antigen by sodium butyrate in PLC/PRF/5 human hepatoma cells". International Journal of Oncology 9.2 (1996): 285-289.
Chicago
Mawatari, F., Nakata, K., Ueki, T., Tsutsumi, T., Ido, A., Nakao, K., Kato, Y., Nagataki, S."Enhanced expression of hepatitis B surface antigen by sodium butyrate in PLC/PRF/5 human hepatoma cells". International Journal of Oncology 9, no. 2 (1996): 285-289. https://doi.org/10.3892/ijo.9.2.285