Resistance to TGF beta in SV40 large T-immortalized rat intestinal epithelial cells is associated with down-regulation of TGF beta type I receptor

  • Authors:
    • Y Mahida
    • S Djelloul
    • A Atfi
    • C Ciacci
    • M Debeaumont
    • S Chevalier
    • C Gespach
    • D Podolsky
  • View Affiliations

  • Published online on: August 1, 1996     https://doi.org/10.3892/ijo.9.2.365
  • Pages: 365-374
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Abstract

A new continuous cell line designated ESKI-1 was established by transfection of rat fetal intestinal epithelial cells with ecotropic retroviruses containing SV40 large T oncogene. The ESKI-1 cell line exhibits morphologic features of an epithelial cell line and expresses the OCI-5 and cytokeratin 8 transcripts associated with epithelial cells in the small intestine. Signal transduction and proliferation responses to TGF beta has been characterized in ESKI-1 cells, in comparison with the spontaneously-immortalized IEC cell lines originating from neonatal rat duodenum and ileum. ESKI-1 express both TGF alpha and TGF beta. However, despite a marked increase in TGF beta-stimulated p78 kinase activity observed in ESKI-1 and IEC cells, TGF beta did not modulate growth, or extracellular matrix expression in ESKI-1 cells. Resistance to growth modulation was associated with downregulation of TGF beta. Type I receptor expression in the SV40 large T-immortalized cells. Thus, proliferative resistance to TGF beta inhibition can result from depletion of the TGF beta type I receptor and disruption of the TGF beta signaling pathway downstream the p78 serine/threonine kinase. These molecular defects constitute two early events during the SV40LT-mediated immortalization and neoplastic progression of the intestinal epithelia.

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August 1996
Volume 9 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mahida Y, Djelloul S, Atfi A, Ciacci C, Debeaumont M, Chevalier S, Gespach C and Podolsky D: Resistance to TGF beta in SV40 large T-immortalized rat intestinal epithelial cells is associated with down-regulation of TGF beta type I receptor. Int J Oncol 9: 365-374, 1996
APA
Mahida, Y., Djelloul, S., Atfi, A., Ciacci, C., Debeaumont, M., Chevalier, S. ... Podolsky, D. (1996). Resistance to TGF beta in SV40 large T-immortalized rat intestinal epithelial cells is associated with down-regulation of TGF beta type I receptor. International Journal of Oncology, 9, 365-374. https://doi.org/10.3892/ijo.9.2.365
MLA
Mahida, Y., Djelloul, S., Atfi, A., Ciacci, C., Debeaumont, M., Chevalier, S., Gespach, C., Podolsky, D."Resistance to TGF beta in SV40 large T-immortalized rat intestinal epithelial cells is associated with down-regulation of TGF beta type I receptor". International Journal of Oncology 9.2 (1996): 365-374.
Chicago
Mahida, Y., Djelloul, S., Atfi, A., Ciacci, C., Debeaumont, M., Chevalier, S., Gespach, C., Podolsky, D."Resistance to TGF beta in SV40 large T-immortalized rat intestinal epithelial cells is associated with down-regulation of TGF beta type I receptor". International Journal of Oncology 9, no. 2 (1996): 365-374. https://doi.org/10.3892/ijo.9.2.365