Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1

  • Authors:
    • U Bergmann
    • H Funatomi
    • M Kornmann
    • T Ishiwata
    • H Beger
    • M Korc
  • View Affiliations

  • Published online on: September 1, 1996     https://doi.org/10.3892/ijo.9.3.487
  • Pages: 487-492
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Abstract

Insulin-like growth factor-II (IGF-II) action and expression were examined in 3 human pancreatic cancer cell lines. IGF-II expression was also studied in 17 normal and 12 malignant pancreatic tissues. IGF-II enhanced the growth of all 3 cell lines. In COLO-357 and PANC-1 cells, one-half maximal stimulation occurred at 0.3 and 0.4 nM IGF-II, respectively. In ASPC-1 cells, one-half maximal stimulation occurred at 0.9 nM IGF-II. A monoclonal antibody (alpha IR3) that blocks ligand binding to the insulin-like growth factor I (IGF-I) receptor (IGF-IR) inhibited IGF-II-mediated growth stimulation, and IGF-II enhanced insulin-receptor substrate 1 (IRS-1) phosphorylation. IGF-II mRNA transcripts were present in COLO-357 cells, in 4 of 17 normal human pancreatic tissues, and in 8 of the 12 cancer samples. By immunohistochemistry, IGF-II was present in the islets of both normal and malignant pancreatic tissues, and occasionally in the cancer cells within the tumor mass. These findings indicate that IGF-II acts via IGF-IR to enhance mitogenic signaling in pancreatic cancer cells and suggest that islet-derived IGF-II may contribute to pancreatic cancer cell growth in vivo.

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September 1996
Volume 9 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Bergmann U, Funatomi H, Kornmann M, Ishiwata T, Beger H and Korc M: Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1. Int J Oncol 9: 487-492, 1996
APA
Bergmann, U., Funatomi, H., Kornmann, M., Ishiwata, T., Beger, H., & Korc, M. (1996). Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1. International Journal of Oncology, 9, 487-492. https://doi.org/10.3892/ijo.9.3.487
MLA
Bergmann, U., Funatomi, H., Kornmann, M., Ishiwata, T., Beger, H., Korc, M."Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1". International Journal of Oncology 9.3 (1996): 487-492.
Chicago
Bergmann, U., Funatomi, H., Kornmann, M., Ishiwata, T., Beger, H., Korc, M."Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1". International Journal of Oncology 9, no. 3 (1996): 487-492. https://doi.org/10.3892/ijo.9.3.487