Enhancement of anti-proliferative activities of 5-FU, HCFU, SN-38, THP and ACNU by a serine protease inhibitor, FOY-305

  • Authors:
    • T Hiwasa
    • H Tokita
    • M Hirose
    • Y Ike
    • M Ohkoshi
  • View Affiliations

  • Published online on: September 1, 1996     https://doi.org/10.3892/ijo.9.3.517
  • Pages: 517-520
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Abstract

The effects of a synthetic serine protease inhibitor, FOY-305, on the proliferation of normal and transformed mouse fibroblasts were investigated in vitro by MTT colorimetric assay. FOY-305 inhibited the growth of normal NIH3T3 cells and their src- and erbB2-transformed cells with a half maximum inhibitory concentration (IC50) of 1-1.2 mg/ml whereas it suppressed the growth of ras-transformed cells more effectively (IC50 was 0.5-0.6 mg/ml). Flow cytometric analysis using synchronized NIH3T3 cells has shown that the growth-inhibitory activity of FOY-305 was due to the suppression of G(1)/S transition. The synergistic effects between FOY-305 and traditional anticancer drugs were also investigated by the MTT assay and the results showed that FOY-305 significantly enhanced the antiproliferative activities of 5-fluorouracil (5-FU), 1-hexylcarbamoyl-5-fluorouracil (HCFU), 7-ethyl-1-hydroxy-7-ethyl-10-[4-(1-piperidino)-1-piperidino] camptothecin (SN-38), pirarubicin (THP) and 1-(4-amino-2-methyl-5-pyrimidinyl)-methyl-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU).

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September 1996
Volume 9 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hiwasa T, Tokita H, Hirose M, Ike Y and Ohkoshi M: Enhancement of anti-proliferative activities of 5-FU, HCFU, SN-38, THP and ACNU by a serine protease inhibitor, FOY-305. Int J Oncol 9: 517-520, 1996
APA
Hiwasa, T., Tokita, H., Hirose, M., Ike, Y., & Ohkoshi, M. (1996). Enhancement of anti-proliferative activities of 5-FU, HCFU, SN-38, THP and ACNU by a serine protease inhibitor, FOY-305. International Journal of Oncology, 9, 517-520. https://doi.org/10.3892/ijo.9.3.517
MLA
Hiwasa, T., Tokita, H., Hirose, M., Ike, Y., Ohkoshi, M."Enhancement of anti-proliferative activities of 5-FU, HCFU, SN-38, THP and ACNU by a serine protease inhibitor, FOY-305". International Journal of Oncology 9.3 (1996): 517-520.
Chicago
Hiwasa, T., Tokita, H., Hirose, M., Ike, Y., Ohkoshi, M."Enhancement of anti-proliferative activities of 5-FU, HCFU, SN-38, THP and ACNU by a serine protease inhibitor, FOY-305". International Journal of Oncology 9, no. 3 (1996): 517-520. https://doi.org/10.3892/ijo.9.3.517