Mutations of p53, E-cadherin, alpha- and beta-catenin genes and tyrosine phosphorylation of beta-catenin in human gastric carcinomas

  • Authors:
    • T Mazaki
    • Y Ishii
    • M Fujii
    • S Iwai
    • K Ishikawa
  • View Affiliations

  • Published online on: September 1, 1996     https://doi.org/10.3892/ijo.9.3.579
  • Pages: 579-583
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Abstract

We investigated whether dysfunction of p53 and E-cadherin participate in invasiveness and metastasis of human gastric carcinoma. We examined twenty-five human gastric carcinomas for p53, E-cadherin, alpha- and beta-catenin gene alteration by the reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) method and sequencing analysis. Three samples (13%) showed p53 gene mutation (two missense mutations and 6 bp deletion). 25% (3/12) of the carcinomas with lymph node metastasis had p53 gene mutations. One sample (4%) showed E-cadherin silent mutation. We were not able to detect alpha- or beta-catenin gene alteration. Therefore we investigated tyrosine-phosphorylation of E-cadherin, a and beta-catenin. Tyrosine-phosphorylated beta-catenin was detected in 13% (2/15) of poorly differentiated carcinomas. These results suggest that the p53 gene mutations have some correlation with lymph node metastasis, and tyrosine phosphorylation of beta-catenin rather than cadherin/catenin gene mutation is at least partly responsible for the loosening of cell-cell contact and invasiveness of poorly differentiated carcinomas.

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September 1996
Volume 9 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mazaki T, Ishii Y, Fujii M, Iwai S and Ishikawa K: Mutations of p53, E-cadherin, alpha- and beta-catenin genes and tyrosine phosphorylation of beta-catenin in human gastric carcinomas. Int J Oncol 9: 579-583, 1996
APA
Mazaki, T., Ishii, Y., Fujii, M., Iwai, S., & Ishikawa, K. (1996). Mutations of p53, E-cadherin, alpha- and beta-catenin genes and tyrosine phosphorylation of beta-catenin in human gastric carcinomas. International Journal of Oncology, 9, 579-583. https://doi.org/10.3892/ijo.9.3.579
MLA
Mazaki, T., Ishii, Y., Fujii, M., Iwai, S., Ishikawa, K."Mutations of p53, E-cadherin, alpha- and beta-catenin genes and tyrosine phosphorylation of beta-catenin in human gastric carcinomas". International Journal of Oncology 9.3 (1996): 579-583.
Chicago
Mazaki, T., Ishii, Y., Fujii, M., Iwai, S., Ishikawa, K."Mutations of p53, E-cadherin, alpha- and beta-catenin genes and tyrosine phosphorylation of beta-catenin in human gastric carcinomas". International Journal of Oncology 9, no. 3 (1996): 579-583. https://doi.org/10.3892/ijo.9.3.579