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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 1996 Volume 9 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 1996 Volume 9 Issue 4

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Article

Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display

  • Authors:
    • M Ikebe
    • B Teicher

  • View Affiliations / Copyright

    Affiliations: DANA FARBER CANC INST,BOSTON,MA 02115. JOINT CTR RADIAT THERAPY,BOSTON,MA 02115.
  • Pages: 629-634
    |
    Published online on: October 1, 1996
       https://doi.org/10.3892/ijo.9.4.629
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Abstract

Differential display was used to define differences in gene expression between murine EMT-6 mammary carcinoma cells grown in monolayer or as spheroids in cell culture. Eighty different combinations of primer sets made of four anchored oligodeo(dT) primers (T(12)MG, T(12)MA, T(12)MT or T(12)MC) and twenty arbitrary ten base primers (AP1-20) were used for RT-PCR of total purified RNA from EMT-6 monolayers or spheroids. After re-screening, fourteen DNA fragments were identified as being selectively expressed in EMT-6 cells grown as spheroids. The fragments were cloned into the pCR II vector. Two of the fourteen cDNA fragments corresponded to mRNA selectively expressed in EMT-6 cells grown as spheroids. These clones were sequenced and found by database searching to correspond to murine heme oxygenase and murine beta(2)-microglobulin. There was a 5.3-fold higher expression of beta(2)-microglobulin and a 5.3-fold greater expression of heme oxygenase in EMT-6 cells grown as spheroids than in the same cells grown as monolayers. These studies reflect the phenotypic flexibility of cells depending upon the growth environment.

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Copy and paste a formatted citation
Spandidos Publications style
Ikebe M and Teicher B: Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display. Int J Oncol 9: 629-634, 1996.
APA
Ikebe, M., & Teicher, B. (1996). Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display. International Journal of Oncology, 9, 629-634. https://doi.org/10.3892/ijo.9.4.629
MLA
Ikebe, M., Teicher, B."Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display". International Journal of Oncology 9.4 (1996): 629-634.
Chicago
Ikebe, M., Teicher, B."Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display". International Journal of Oncology 9, no. 4 (1996): 629-634. https://doi.org/10.3892/ijo.9.4.629
Copy and paste a formatted citation
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Spandidos Publications style
Ikebe M and Teicher B: Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display. Int J Oncol 9: 629-634, 1996.
APA
Ikebe, M., & Teicher, B. (1996). Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display. International Journal of Oncology, 9, 629-634. https://doi.org/10.3892/ijo.9.4.629
MLA
Ikebe, M., Teicher, B."Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display". International Journal of Oncology 9.4 (1996): 629-634.
Chicago
Ikebe, M., Teicher, B."Alterations in gene expression between EMT-6 mammary carcinoma monolayers and spheroids identified by differential display". International Journal of Oncology 9, no. 4 (1996): 629-634. https://doi.org/10.3892/ijo.9.4.629
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