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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2008 Volume 33 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2008 Volume 33 Issue 3

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Article

Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide

  • Authors:
    • Francesca Donà
    • Ennio Prosperi
    • Monica Savio
    • Tania Coppa
    • A. Ivana Scovassi
    • Chiara Mondello

  • View Affiliations / Copyright

    Affiliations: Istituto di Genetica Molecolare CNR, I-27100 Pavia, Italy
  • Pages: 613-621
    |
    Published online on: September 1, 2008
       https://doi.org/10.3892/ijo_00000047
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Abstract

In mammalian cells, the H2AX histone is rapidly phosphorylated upon the induction of DNA double strand breaks and promotes their repair, which is required for preserving genomic integrity. Etoposide is an inhibitor of DNA topoisomerase II, which causes DNA breaks and induces H2AX phosphorylation. To elucidate whether H2AX may affect cellular sensitivity to etoposide, we studied the response to this agent in immortalized embryonic fibroblasts derived from H2AX knockout mice. Clonogenic assays in cells treated with the drug revealed a greater sensitivity of H2AX null cells compared to wild-type cells, possibly due to the persistence of a higher number of DNA breaks, as detected with the comet assay. In both cell lines, etoposide induced micronuclei formation and nuclear fragmentation; however, in H2AX deficient cells nuclear fragmentation was observed at a lower drug concentration. Flow cytometric analysis showed that etoposide induced a G2/M cell cycle arrest in both cell lines, which occurred at lower drug concentrations in H2AX deficient cells. G2/M arrest was paralleled by an accumulation of cyclin A and cyclin B1, suggesting that treated cells are not able to complete cell cycle correctly and undergo cell death. Taken together, our observations suggest that H2AX takes part to the cellular response to etoposide and confirm its role in the maintenance of genome stability.

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Copy and paste a formatted citation
Spandidos Publications style
Donà F, Prosperi E, Savio M, Coppa T, Scovassi AI and Mondello C: Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide. Int J Oncol 33: 613-621, 2008.
APA
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A.I., & Mondello, C. (2008). Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide. International Journal of Oncology, 33, 613-621. https://doi.org/10.3892/ijo_00000047
MLA
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A. I., Mondello, C."Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide". International Journal of Oncology 33.3 (2008): 613-621.
Chicago
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A. I., Mondello, C."Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide". International Journal of Oncology 33, no. 3 (2008): 613-621. https://doi.org/10.3892/ijo_00000047
Copy and paste a formatted citation
x
Spandidos Publications style
Donà F, Prosperi E, Savio M, Coppa T, Scovassi AI and Mondello C: Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide. Int J Oncol 33: 613-621, 2008.
APA
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A.I., & Mondello, C. (2008). Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide. International Journal of Oncology, 33, 613-621. https://doi.org/10.3892/ijo_00000047
MLA
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A. I., Mondello, C."Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide". International Journal of Oncology 33.3 (2008): 613-621.
Chicago
Donà, F., Prosperi, E., Savio, M., Coppa, T., Scovassi, A. I., Mondello, C."Loss of histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase II inhibitor etoposide". International Journal of Oncology 33, no. 3 (2008): 613-621. https://doi.org/10.3892/ijo_00000047
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