Open Access

Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines

  • Authors:
    • Dimitrios J. Stravopodis
    • Panagiotis K. Karkoulis
    • Eumorphia G. Konstantakou
    • Sophia Melachroinou
    • Antonis D. Lampidonis
    • Dimitra Anastasiou
    • Stefanos Kachrilas
    • Niki Messini-Nikolaki
    • Issidora S. Papassideri
    • Gerasimos Aravantinos
    • Lukas H. Margaritis
    • Gerassimos E. Voutsinas
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/ijo_00000137
  • Pages: 137-160
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Doxorubicin is an important component of combination therapy for muscle-invasive urinary bladder cancer. Treatment with this topoisomerase II poison is able to interfere with cell cycle progression and lead to cancer cell death. Using FACS analysis, Western immunoblotting and semi-quantitative RT-PCR, we studied the effects of doxorubicin on cell cycle progression and apoptosis, and also explored the possibility of using groups of genes as biomarkers of prognosis and/or response to doxorubicin treatment in human urinary bladder cancer cells. Doxorubicin induced dose-dependent G2/M and/or G1/S cell cycle arrest, followed by grade- and dose-dependent reduction in the amount of the cytosolic trimeric form of FasL, activation of Caspase-8, Caspase-9, Caspase-3, cleavage of PARP, Lamin A/C, Bcl-XL/S and interestingly Hsp90, and finally cell death. Data presented here also suggest the use of the expression patterns of Cyclin-E2, Cyclin-F, p63, p73, FasL, TRAIL, Tweak, Tweak-R, XAF-1, OPG and Bok genes for identification of the differentiation grade, and Cyclin-B2, GADD45A, p73, FasL, Bik, Bim, TRAIL, Fas, Tweak-R, XAF-1, Bcl-2, Survivin, OPG, DcR2 and Bcl-XL genes for the detection of response to doxorubicin in human bladder cancer cells.

Related Articles

Journal Cover

January 2009
Volume 34 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Stravopodis DJ, Karkoulis PK, Konstantakou EG, Melachroinou S, Lampidonis AD, Anastasiou D, Kachrilas S, Messini-Nikolaki N, Papassideri IS, Aravantinos G, Aravantinos G, et al: Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines. Int J Oncol 34: 137-160, 2009
APA
Stravopodis, D.J., Karkoulis, P.K., Konstantakou, E.G., Melachroinou, S., Lampidonis, A.D., Anastasiou, D. ... Voutsinas, G.E. (2009). Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines. International Journal of Oncology, 34, 137-160. https://doi.org/10.3892/ijo_00000137
MLA
Stravopodis, D. J., Karkoulis, P. K., Konstantakou, E. G., Melachroinou, S., Lampidonis, A. D., Anastasiou, D., Kachrilas, S., Messini-Nikolaki, N., Papassideri, I. S., Aravantinos, G., Margaritis, L. H., Voutsinas, G. E."Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines". International Journal of Oncology 34.1 (2009): 137-160.
Chicago
Stravopodis, D. J., Karkoulis, P. K., Konstantakou, E. G., Melachroinou, S., Lampidonis, A. D., Anastasiou, D., Kachrilas, S., Messini-Nikolaki, N., Papassideri, I. S., Aravantinos, G., Margaritis, L. H., Voutsinas, G. E."Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines". International Journal of Oncology 34, no. 1 (2009): 137-160. https://doi.org/10.3892/ijo_00000137