HPV 16-associated tumours: IL-12 can repair the absence of cytotoxic and proliferative responses of tumour infiltrating cells after chemotherapy

  • Authors:
    • Marie Indrová
    • Jana Bieblová
    • Joana Rossowska
    • Piotr Kuropka
    • Elzbieta Pajtasz-Piasecka
    • Jan Bubeník
    • Milan Reinis
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/ijo_00000139
  • Pages: 173-179
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Abstract

We have examined the effect of IL-12-producing cellular vaccines on the cytotoxicity and proliferative potential of CD45+ tumour-infiltrating cells (TIL) in mice carrying syngeneic TC-1 and TC-1/A9 HPV 16-associated tumours after chemotherapy with CBM-4A ifosfamide derivative. The chemotherapy resulted in the decrease of the CD4+ and CD8+ TIL, increase of the Gr-1+/CD11b+ TIL, no changes in the infiltration with CD4+/CD25+ Treg TIL, and decrease of the cytolytic and proliferative potential of the CD45+ TIL. Subsequent immunotherapy with the IL-12-producing, genetically modified TC-1 (TC-1-IL-12) cells increased tumour infiltration with CD8+ and CD4+ cells, decreased the Gr-1+/CD11b+ cells, and increased the cytolytic and proliferative potential of the CD45+ TIL. Taken together, these findings suggest that peritumoral administration of the IL-12-producing cellular vaccine can restore the cytolytic potential and inhibit immunosuppressive TIL-dependent mechanisms in the individuals bearing HPV 16-associated tumours, and explain our previously described tumour-inhibitory effects of the vaccine in mice with minimal residual disease after the tumour chemotherapy.

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January 2009
Volume 34 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Indrová M, Bieblová J, Rossowska J, Kuropka P, Pajtasz-Piasecka E, Bubeník J and Reinis M: HPV 16-associated tumours: IL-12 can repair the absence of cytotoxic and proliferative responses of tumour infiltrating cells after chemotherapy. Int J Oncol 34: 173-179, 2009
APA
Indrová, M., Bieblová, J., Rossowska, J., Kuropka, P., Pajtasz-Piasecka, E., Bubeník, J., & Reinis, M. (2009). HPV 16-associated tumours: IL-12 can repair the absence of cytotoxic and proliferative responses of tumour infiltrating cells after chemotherapy. International Journal of Oncology, 34, 173-179. https://doi.org/10.3892/ijo_00000139
MLA
Indrová, M., Bieblová, J., Rossowska, J., Kuropka, P., Pajtasz-Piasecka, E., Bubeník, J., Reinis, M."HPV 16-associated tumours: IL-12 can repair the absence of cytotoxic and proliferative responses of tumour infiltrating cells after chemotherapy". International Journal of Oncology 34.1 (2009): 173-179.
Chicago
Indrová, M., Bieblová, J., Rossowska, J., Kuropka, P., Pajtasz-Piasecka, E., Bubeník, J., Reinis, M."HPV 16-associated tumours: IL-12 can repair the absence of cytotoxic and proliferative responses of tumour infiltrating cells after chemotherapy". International Journal of Oncology 34, no. 1 (2009): 173-179. https://doi.org/10.3892/ijo_00000139