In vitro anti-cancer activity of two ethno-pharmacological healing plants from Guatemala Pluchea odorata and Phlebodium decumanum

  • Authors:
    • Manuela Gridling
    • Nicole Stark
    • Sibylle Madlener
    • Andreas Lackner
    • Ruxandra Popescu
    • Birgit Benedek
    • Rene Diaz
    • Foster M. Tut
    • Thanh Phuong Nha Vo
    • Daniela Huber
    • Michaela Gollinger
    • Philipp Saiko
    • Ali Özmen
    • Wilhelm Mosgoeller
    • Rainer De Martin
    • Ruth Eytner
    • Karl-Heinz Wagner
    • Michael Grusch
    • Monika Fritzer-Szekeres
    • Thomas Szekeres
    • Brigitte Kopp
    • Richard Frisch
    • Georg Krupitza
  • View Affiliations

  • Published online on: April 1, 2009     https://doi.org/10.3892/ijo_00000239
  • Pages: 1117-1128
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Abstract

Many traditional healing plants successfully passed several hundred years of empirical testing against specific diseases and thereby demonstrating that they are well tolerated in humans. Although quite a few ethno-pharmacological plants are applied against a variety of conditions there are still numerous plants that have not been cross-tested in diseases apart from the traditional applications. Herein we demonstrate the anti-neoplastic potential of two healing plants used by the Maya of the Guatemala/Belize area against severe inflammatory conditions such as neuritis, rheumatism, arthritis, coughs, bruises and tumours. Phlebodium decumanum and Pluchea odorata were collected, dried and freeze dried, and extracted with five solvents of increasing polarity. We tested HL-60 and MCF-7 cells, the inhibition of proliferation and the induction of cell death were investigated as hallmark endpoints to measure the efficiency of anti-cancer drugs. Western blot and FACS analyses elucidated the underlying mechanisms. While extracts of P. decumanum showed only moderate anti-cancer activity and were therefore not further analysed, particularly the dichloromethane extract of P. odorata inhibited the cell cycle in G2-M which correlated with the activation of checkpoint kinase 2, and down-regulation of Cdc25A and cyclin D1 as well as inactivation of Erk1/2. In HL-60 and MCF-7 cells this extract was a very strong inducer of cell death activating caspase-3 followed by PARP signature type cleavage. The initiating death trigger was likely the stabilization of microtubules monitored by the rapid acetylation of α-tubulin, which was even more pronounced than that triggered by taxol. The dichloromethane extract of P. odorata contains apolar constituents which inhibit inflam-matory responses and exhibit anti-cancer activity. The strong proapoptotic potential warrants further bioassay-guided fractionation to discover and test the active principle(s).

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April 2009
Volume 34 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Gridling, M., Stark, N., Madlener, S., Lackner, A., Popescu, R., Benedek, B. ... Krupitza, G. (2009). In vitro anti-cancer activity of two ethno-pharmacological healing plants from Guatemala Pluchea odorata and Phlebodium decumanum. International Journal of Oncology, 34, 1117-1128. https://doi.org/10.3892/ijo_00000239
MLA
Gridling, M., Stark, N., Madlener, S., Lackner, A., Popescu, R., Benedek, B., Diaz, R., Tut, F. M., Nha Vo, T. P., Huber, D., Gollinger, M., Saiko, P., Özmen, A., Mosgoeller, W., De Martin, R., Eytner, R., Wagner, K., Grusch, M., Fritzer-Szekeres, M., Szekeres, T., Kopp, B., Frisch, R., Krupitza, G."In vitro anti-cancer activity of two ethno-pharmacological healing plants from Guatemala Pluchea odorata and Phlebodium decumanum". International Journal of Oncology 34.4 (2009): 1117-1128.
Chicago
Gridling, M., Stark, N., Madlener, S., Lackner, A., Popescu, R., Benedek, B., Diaz, R., Tut, F. M., Nha Vo, T. P., Huber, D., Gollinger, M., Saiko, P., Özmen, A., Mosgoeller, W., De Martin, R., Eytner, R., Wagner, K., Grusch, M., Fritzer-Szekeres, M., Szekeres, T., Kopp, B., Frisch, R., Krupitza, G."In vitro anti-cancer activity of two ethno-pharmacological healing plants from Guatemala Pluchea odorata and Phlebodium decumanum". International Journal of Oncology 34, no. 4 (2009): 1117-1128. https://doi.org/10.3892/ijo_00000239