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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2009 Volume 34 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2009 Volume 34 Issue 5

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1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo

  • Authors:
    • Hiromi Kazuno
    • Akio Fujioka
    • Masakazu Fukushima
    • Yusuke Wataya
    • Akira Matsuda
    • Takuma Sasaki
  • View Affiliations / Copyright

    Affiliations: Hanno Research Center, Taiho Pharmaceutical Co., Ltd., Hanno-city, Saitama 357-8527, Japan. h-kazuno@taiho.co.jp
  • Pages: 1373-1380
    |
    Published online on: May 1, 2009
       https://doi.org/10.3892/ijo_00000264
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Abstract

1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106) is a novel antitumor ribonucleoside that inhibits RNA polymerase. In the present study, we investigated the cellular and molecular interactions between TAS-106 and cisplatin (CDDP) in vitro using A549 human lung cancer cells and the in vivo antitumor effect of combined treatment using OCC-1 and LX-1 human tumor xenografts. The treatment effects were determined by evaluating cytotoxicity, the cell cycle distribution, apoptosis induction and the expression of checkpoint-associated proteins. In vitro, the combination of TAS-106 and CDDP synergistically inhibited the growth of A549 cells, as determined using isobologram analysis. TAS-106 potently inhibited the expression of Chk1 protein and the phosphorylation of Chk1 and Chk2. Moreover, based on the inhibition of checkpoint-associated protein, TAS-106 abrogated the CDDP-induced S- and G2M-checkpoints and induced apoptosis in A549 cells. In vivo, TAS-106 alone showed antitumor activity; however, its combination with CDDP significantly enhanced the growth inhibition of OCC-1 and LX-1 tumors. Moreover, combination therapy with TAS-106 and CDDP in the OCC-1 xenograft model resulted in significant life-prolongation. These findings provide a rationale for combination chemotherapy using TAS-106 and CDDP in clinical settings.

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Copy and paste a formatted citation
Spandidos Publications style
Kazuno H, Fujioka A, Fukushima M, Wataya Y, Matsuda A and Sasaki T: 1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo. Int J Oncol 34: 1373-1380, 2009.
APA
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., & Sasaki, T. (2009). 1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo. International Journal of Oncology, 34, 1373-1380. https://doi.org/10.3892/ijo_00000264
MLA
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., Sasaki, T."1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo". International Journal of Oncology 34.5 (2009): 1373-1380.
Chicago
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., Sasaki, T."1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo". International Journal of Oncology 34, no. 5 (2009): 1373-1380. https://doi.org/10.3892/ijo_00000264
Copy and paste a formatted citation
x
Spandidos Publications style
Kazuno H, Fujioka A, Fukushima M, Wataya Y, Matsuda A and Sasaki T: 1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo. Int J Oncol 34: 1373-1380, 2009.
APA
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., & Sasaki, T. (2009). 1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo. International Journal of Oncology, 34, 1373-1380. https://doi.org/10.3892/ijo_00000264
MLA
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., Sasaki, T."1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo". International Journal of Oncology 34.5 (2009): 1373-1380.
Chicago
Kazuno, H., Fujioka, A., Fukushima, M., Wataya, Y., Matsuda, A., Sasaki, T."1-(3-C-Ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS-106), a novel potent inhibitor of RNA polymerase, potentiates the cytotoxicity of CDDP in human cancer cells both in vitro and in vivo". International Journal of Oncology 34, no. 5 (2009): 1373-1380. https://doi.org/10.3892/ijo_00000264
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