Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells

  • Authors:
    • Hsiu-Maan Kuo
    • Hung-Chieh Tsai
    • Ya-Ling Lin
    • Jai-Sing Yang
    • An-Cheng Huang
    • Mei-Due Yang
    • Shu-Chun Hsu
    • Meng-Chin Chung
    • W. Gibson Wood
    • Jing-Gung Chung
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000384
  • Pages: 717-724
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Abstract

Baicalein has been reported to induce growth-inhibitory activity in vitro in human cancer cells; however, the molecular mechanism of action is not completely understood. A pharmacological dose (10-100 µM) of baicalein exerted a cytotoxic effect on human hepatoma J5 cells resulting in G2/M arrest and apoptosis. In addition to cytotoxicity in J5 cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9 and -3 occurred. Baicalein induced AIF and Endo G release from mitochondria indicating that baicalein stimulates apoptosis through the caspase-independent pathway, while undergoing apoptosis, there was a remarkable accumulation of G2/M cells. Also, the ratio of Bax/Bcl-2 was increased leading to changes in mitochondria membrane potential (ΔΨm) and release of cytochrome c, whereas the baicalein-induced apoptosis was partially abrogated by pretreatment with the pan-caspase inhibitor z-VAD-fmk, the accumulation of G2/M cells remained. These results demonstrate that the cytotoxicity of baicalein in J5 cells is attributable to apoptosis mainly involving G2/M-arrest in an ER-dependent manner, via a mitochondria-dependent caspase pathway and as well as contributions of AIF and Endo G pathways.

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October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kuo H, Tsai H, Lin Y, Yang J, Huang A, Yang M, Hsu S, Chung M, Gibson Wood W, Chung J, Chung J, et al: Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells. Int J Oncol 35: 717-724, 2009.
APA
Kuo, H., Tsai, H., Lin, Y., Yang, J., Huang, A., Yang, M. ... Chung, J. (2009). Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells. International Journal of Oncology, 35, 717-724. https://doi.org/10.3892/ijo_00000384
MLA
Kuo, H., Tsai, H., Lin, Y., Yang, J., Huang, A., Yang, M., Hsu, S., Chung, M., Gibson Wood, W., Chung, J."Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells". International Journal of Oncology 35.4 (2009): 717-724.
Chicago
Kuo, H., Tsai, H., Lin, Y., Yang, J., Huang, A., Yang, M., Hsu, S., Chung, M., Gibson Wood, W., Chung, J."Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells". International Journal of Oncology 35, no. 4 (2009): 717-724. https://doi.org/10.3892/ijo_00000384