Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma

  • Authors:
    • Chueh-Chuan Yen
    • Wei-Ming Chen
    • Tain-Hsiung Chen
    • Winby York-Kwan Chen
    • Paul Chih-Hsueh Chen
    • Hong-Jen Chiou
    • Giun-Yi Hung
    • Hung-Ta Hondar Wu
    • Chao-Jung Wei
    • Cheng-Ying Shiau
    • Yu-Chung Wu
    • Ta-Chung Chao
    • Cheng-Hwai Tzeng
    • Po-Min Chen
    • Chi-Hung Lin
    • Yann-Jang Chen
    • Jonathan A. Fletcher
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000390
  • Pages: 775-788
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Five osteosarcoma (OS) cell lines, 37 OS tumors and 9 corresponding non-neoplastic samples were genotyped by Affymetrix 10 K 2.0 SNP array. Regions of high level amplification and homozygous deletion were identified and validated by quantitative PCR and FISH. Certain recurrent cytogenetic alterations were more frequent in recurrent/metastatic than in primary OS. These included deletion of 6q14.1, 6q16.2-q22.31, and 8p23.2-p12, amplification of 8q21.12, 8q22.3-q24.3 and 17p12, and loss of heterozygosity (LOH) at 2q24.3-q31.2, 5q11.2, 6p21.31-p21.1, 6q14.1-q16.2, 8p22-p12, 9q22.1, 10q21.1-q22.1, 10q23.31-q24.1, 12q15-q21.1 and 21q21.2-q21.3. Most of the LOH calls were associated with deletion, but a subset of them was associated with normal or increased copy number (CN). A consensus 3q13.31 deletion localized to a region within the limbic system-associated membrane protein (LSAMP) gene was also identified. The FISH evaluations demonstrated highly-localized homozygous or heterozygous LSAMP deletions in 6 of 11 primary OS. qRT-PCR evaluations of the two major alternative LSAMP transcripts demonstrated reduced expression of 1b isoform transcript in each of three OS with LSAMP exon 1b deletion. Further, the 1a isoform transcripts in these same OS had either reduced expression or a premature termination codon in LSAMP exon 2. This SNP genotyping study identified chromosomal aberrations associated with disease progression in OS and disclosed LSAMP as a novel tumor suppressor gene in OS. The study also demonstrated that CN and LOH analyses were able to detect distinct subsets of genetic abnormalities in OS.

Related Articles

Journal Cover

October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yen C, Chen W, Chen T, Chen WY, Chen PC, Chiou H, Hung G, Wu HH, Wei C, Shiau C, Shiau C, et al: Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma. Int J Oncol 35: 775-788, 2009
APA
Yen, C., Chen, W., Chen, T., Chen, W.Y., Chen, P.C., Chiou, H. ... Fletcher, J.A. (2009). Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma. International Journal of Oncology, 35, 775-788. https://doi.org/10.3892/ijo_00000390
MLA
Yen, C., Chen, W., Chen, T., Chen, W. Y., Chen, P. C., Chiou, H., Hung, G., Wu, H. H., Wei, C., Shiau, C., Wu, Y., Chao, T., Tzeng, C., Chen, P., Lin, C., Chen, Y., Fletcher, J. A."Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma". International Journal of Oncology 35.4 (2009): 775-788.
Chicago
Yen, C., Chen, W., Chen, T., Chen, W. Y., Chen, P. C., Chiou, H., Hung, G., Wu, H. H., Wei, C., Shiau, C., Wu, Y., Chao, T., Tzeng, C., Chen, P., Lin, C., Chen, Y., Fletcher, J. A."Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma". International Journal of Oncology 35, no. 4 (2009): 775-788. https://doi.org/10.3892/ijo_00000390