SiRNA of Frizzled-9 suppresses proliferation and motility of hepatoma cells

  • Authors:
    • Tatsuya Fujimoto
    • Minoru Tomizawa
    • Osamu Yokosuka
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000400
  • Pages: 861-866
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Frizzled (Fz), a receptor of Wnt ligands, plays key roles in liver carcinogenesis. Its expression was analyzed as part of a search for a target of molecular therapy for hepatocellular carcinoma (HCC) and hepatoblastoma (HB). Fz genes were analyzed by RT-PCR in HCC cell lines HLE, HLF, PLC/PRF/5, Huh-7 and Hep3B, HB cell lines Huh-6 and HepG2, HeLa cells, human normal fetal and adult liver. We transfected PLC/PRF/5, HLE, Huh-6, and HeLa cells with Fz9-small interfering RNA (Fz9-siRNA). Five days after transfection, cell proliferation was analyzed by MTS assay and cell motility by wound assay with H&E staining. Subsequently, the expressions of cyclin D1 and caspase-3 were analyzed by Western blot analysis. Fz9-siRNA decreased the expression of Fz9 gene in all cell lines. MTS assay showed that Fz9-siRNA significantly suppressed cell proliferation and cell motility in all cell lines. The expression of cyclin D1 was also suppressed by Western blotting. Cleaved caspase-3 did not appear and apoptosis was not observed in any of the cell lines tested. We demonstrated that Fz9 plays an essential role in carcinogenesis of HB and HCC, concluding that Fz9-siRNA could represent a useful therapeutic target for HB and HCC.

Related Articles

Journal Cover

October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Fujimoto T, Tomizawa M and Yokosuka O: SiRNA of Frizzled-9 suppresses proliferation and motility of hepatoma cells. Int J Oncol 35: 861-866, 2009
APA
Fujimoto, T., Tomizawa, M., & Yokosuka, O. (2009). SiRNA of Frizzled-9 suppresses proliferation and motility of hepatoma cells. International Journal of Oncology, 35, 861-866. https://doi.org/10.3892/ijo_00000400
MLA
Fujimoto, T., Tomizawa, M., Yokosuka, O."SiRNA of Frizzled-9 suppresses proliferation and motility of hepatoma cells". International Journal of Oncology 35.4 (2009): 861-866.
Chicago
Fujimoto, T., Tomizawa, M., Yokosuka, O."SiRNA of Frizzled-9 suppresses proliferation and motility of hepatoma cells". International Journal of Oncology 35, no. 4 (2009): 861-866. https://doi.org/10.3892/ijo_00000400