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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April 2010 Volume 36 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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April 2010 Volume 36 Issue 4

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Article

Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo

  • Authors:
    • Satoshi Takenaka
    • Norifumi Naka
    • Nobuhito Araki
    • Nobuyuki Hashimoto
    • Takafumi Ueda
    • Kiyoko Yoshioka
    • Hideki Yoshikawa
    • Kazuyuki Itoh
  • View Affiliations / Copyright

    Affiliations: Department of Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka 537-8511, Japan
  • Pages: 823-831
    |
    Published online on: April 1, 2010
       https://doi.org/10.3892/ijo_00000559
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Abstract

Synovial sarcoma (SS) is an aggressive soft-tissue malignancy characterized by a unique t(X;18) translocation resulting in expression of SS18-SSX fusion protein. In order to investigate the biological function of this fusion protein and to develop a novel therapeutic option, we examined downregulation of SS18-SSX1 expression by small interfering RNA targeting SS18-SSX1 in three human SS cell lines. Microarray analysis comparing SS18-SSX1-silenced cells with control cells in three SS cell lines showed that SS18-SSX1 mainly affected the focal adhesion pathway. In accord with the array data, silencing of SS18-SSX1 enhances adhesion to the extracellular matrix through the induction of expression of myosin light-chain kinase. Furthermore, the silencing of SS18-SSX1 inhibits anchorage-independent growth in vitro and systemic delivery of siRNA against SS18-SSX1 using a nanoparticle system inhibited tumor growth in a nude mouse xenograft model. Our results demonstrate that siRNA targeting of SS18-SSX1 has therapeutic potential for the treatment of SS.

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Copy and paste a formatted citation
Spandidos Publications style
Takenaka S, Naka N, Araki N, Hashimoto N, Ueda T, Yoshioka K, Yoshikawa H and Itoh K: Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. Int J Oncol 36: 823-831, 2010.
APA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K. ... Itoh, K. (2010). Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. International Journal of Oncology, 36, 823-831. https://doi.org/10.3892/ijo_00000559
MLA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36.4 (2010): 823-831.
Chicago
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36, no. 4 (2010): 823-831. https://doi.org/10.3892/ijo_00000559
Copy and paste a formatted citation
x
Spandidos Publications style
Takenaka S, Naka N, Araki N, Hashimoto N, Ueda T, Yoshioka K, Yoshikawa H and Itoh K: Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. Int J Oncol 36: 823-831, 2010.
APA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K. ... Itoh, K. (2010). Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. International Journal of Oncology, 36, 823-831. https://doi.org/10.3892/ijo_00000559
MLA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36.4 (2010): 823-831.
Chicago
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36, no. 4 (2010): 823-831. https://doi.org/10.3892/ijo_00000559
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