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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2010 Volume 36 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article Open Access

Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2

  • Authors:
    • Birgitte B. Olsen
    • Jan N. Kreutzer
    • Nobumoto Watanabe
    • Tina Holm
    • Barbara Guerra

  • View Affiliations / Copyright

    Affiliations: Institute of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark
  • Pages: 1175-1182
    |
    Published online on: May 1, 2010
       https://doi.org/10.3892/ijo_00000600
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Abstract

Somatic Wee1 is a protein kinase that plays a key role in cell cycle progression at the onset of mitosis by phosphorylating CDK1 at the inhibitory Tyr15 amino acid residue. Wee1 is regulated at multiple levels, i.e., phosphorylation, protein-protein association and proteasome-mediated degradation. We have recently shown that the regulatory β-subunit of protein kinase CK2 participates in PLK1-Wee1 complex formation interacting directly with Wee1 and thereby contributing to the regulation of G2-M cell cycle transition. Here, we show that Wee1 binds CK2β via two domains comprising amino acids 59-71 and 232-332. By employing deletion mutants of the CK2β-subunit we also show that two regions between residues 1-5 and 155-170 are necessary for binding Wee1. Furthermore, we demonstrate that the interaction between CK2β and Wee1 does not modify the kinase activity of the latter, instead CK2β directly upregulates CDK1 kinase activity by reversing the inhibitory effect which follows Wee1-mediated phosphorylation. Taken together, our findings reinforce the notion that CK2β is a modulator of protein kinases implicated in cell cycle regulation and exerts functions that are independent of CK2 tetramers.

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Copy and paste a formatted citation
Spandidos Publications style
Olsen BB, Kreutzer JN, Watanabe N, Holm T and Guerra B: Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2. Int J Oncol 36: 1175-1182, 2010.
APA
Olsen, B.B., Kreutzer, J.N., Watanabe, N., Holm, T., & Guerra, B. (2010). Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2. International Journal of Oncology, 36, 1175-1182. https://doi.org/10.3892/ijo_00000600
MLA
Olsen, B. B., Kreutzer, J. N., Watanabe, N., Holm, T., Guerra, B."Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2". International Journal of Oncology 36.5 (2010): 1175-1182.
Chicago
Olsen, B. B., Kreutzer, J. N., Watanabe, N., Holm, T., Guerra, B."Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2". International Journal of Oncology 36, no. 5 (2010): 1175-1182. https://doi.org/10.3892/ijo_00000600
Copy and paste a formatted citation
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Spandidos Publications style
Olsen BB, Kreutzer JN, Watanabe N, Holm T and Guerra B: Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2. Int J Oncol 36: 1175-1182, 2010.
APA
Olsen, B.B., Kreutzer, J.N., Watanabe, N., Holm, T., & Guerra, B. (2010). Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2. International Journal of Oncology, 36, 1175-1182. https://doi.org/10.3892/ijo_00000600
MLA
Olsen, B. B., Kreutzer, J. N., Watanabe, N., Holm, T., Guerra, B."Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2". International Journal of Oncology 36.5 (2010): 1175-1182.
Chicago
Olsen, B. B., Kreutzer, J. N., Watanabe, N., Holm, T., Guerra, B."Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2". International Journal of Oncology 36, no. 5 (2010): 1175-1182. https://doi.org/10.3892/ijo_00000600
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