Open Access

Identification of autoantibody against fatty acid synthase in hepatocellular carcinoma mouse model and its application to diagnosis of HCC

  • Authors:
    • Chang-Kyu Heo
    • Mi-Kyung Woo
    • Dae-Yeul Yu
    • Ju Yeon Lee
    • Jong Shin Yoo
    • Hyang Sook Yoo
    • Jeong Heon Ko
    • Jin-Man Kim
    • Jong Young Choi
    • In Gyu Kim
    • Sang Gi Paik
    • Eun-Wie Cho
  • View Affiliations

  • Published online on: June 1, 2010     https://doi.org/10.3892/ijo_00000631
  • Pages: 1453-1459
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Autoantibodies, which are generated by immune system recognizing the presence of the abnormal tumor-associated antigens, are promising biomarkers for early detection of tumors. Recently, we established a B cell hybridoma pool derived from H-ras12V transgenic mouse, a typical hepatocellular carcinoma model, as a source of tumor-associated autoantibodies without using any extracellular antigens and have characterized the specific target antigens against them. K1 autoantibody, one of them, was investigated in this study and its target antigen was identified by mass spectrometric analysis as fatty acid synthase (FASN), an important oncogenic protein. Moreover, a specific mimotope against K1 autoantibody was screened from the cyclic random hepta-peptide phage library and, using it as a coating antigen for ELISA, we could distinguish patients with hepatocellular carcinoma (HCC) vs. normal subjects with 96.55% sensitivity and 100% specificity. These results imply that anti-FASN autoantibody is induced in patients with HCC and detection of anti-FASN autoantibody can be used for the diagnosis of HCC.

Related Articles

Journal Cover

June 2010
Volume 36 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Heo C, Woo M, Yu D, Lee JY, Yoo JS, Yoo HS, Ko JH, Kim J, Choi JY, Kim IG, Kim IG, et al: Identification of autoantibody against fatty acid synthase in hepatocellular carcinoma mouse model and its application to diagnosis of HCC. Int J Oncol 36: 1453-1459, 2010.
APA
Heo, C., Woo, M., Yu, D., Lee, J.Y., Yoo, J.S., Yoo, H.S. ... Cho, E. (2010). Identification of autoantibody against fatty acid synthase in hepatocellular carcinoma mouse model and its application to diagnosis of HCC. International Journal of Oncology, 36, 1453-1459. https://doi.org/10.3892/ijo_00000631
MLA
Heo, C., Woo, M., Yu, D., Lee, J. Y., Yoo, J. S., Yoo, H. S., Ko, J. H., Kim, J., Choi, J. Y., Kim, I. G., Paik, S. G., Cho, E."Identification of autoantibody against fatty acid synthase in hepatocellular carcinoma mouse model and its application to diagnosis of HCC". International Journal of Oncology 36.6 (2010): 1453-1459.
Chicago
Heo, C., Woo, M., Yu, D., Lee, J. Y., Yoo, J. S., Yoo, H. S., Ko, J. H., Kim, J., Choi, J. Y., Kim, I. G., Paik, S. G., Cho, E."Identification of autoantibody against fatty acid synthase in hepatocellular carcinoma mouse model and its application to diagnosis of HCC". International Journal of Oncology 36, no. 6 (2010): 1453-1459. https://doi.org/10.3892/ijo_00000631