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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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June 2010 Volume 36 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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June 2010 Volume 36 Issue 6

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Article

Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells

  • Authors:
    • Chunzhi Zhang
    • Lei Han
    • Anling Zhang
    • Weidong Yang
    • Xuan Zhou
    • Peiyu Pu
    • Yue Du
    • Huazong Zeng
    • Chunsheng Kang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
  • Pages: 1503-1512
    |
    Published online on: June 1, 2010
       https://doi.org/10.3892/ijo_00000637
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Abstract

MicroRNA (miR)-221 and miR-222 are frequently upregulated in various types of human malignancy including glioblastoma. Previous studies have identified some targets of miR-221 and miR-222, such as p27 and p57. Inter-relationship between miR-221 and miR-222 expression and global mRNA expression remains elusive. Here we knocked down miR-221 and miR-222 expression and found 158 differentially expressed genes with 2-fold changes in U251 glioma cells by microarray analysis. Using the KEGG pathway databases and BioCarta, we found that the IFN-α signaling pathway was the most significant pathway modulated by differentially expressed genes. STAT1 and STAT2 are core proteins in the IFN-α signaling pathway. By Western blotting and immunofluorescence, we found that STAT1 and STAT2 expression and phosphorylation were upregulated in U251 cells with knocked-down miR-221/222. Furthermore, tyrosine phosphorylation of STAT1 and STAT2 was present in the nucleus after repression of miR-221/222 expression in U251 cells. These data indicate for the first time a mechanism involving STAT1/2 upregulation under the transcriptional control of INF-α signaling after knockdown of miR-221/222 cluster in U251 glioma cells.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Han L, Zhang A, Yang W, Zhou X, Pu P, Du Y, Zeng H and Kang C: Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells. Int J Oncol 36: 1503-1512, 2010.
APA
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P. ... Kang, C. (2010). Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells. International Journal of Oncology, 36, 1503-1512. https://doi.org/10.3892/ijo_00000637
MLA
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P., Du, Y., Zeng, H., Kang, C."Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells". International Journal of Oncology 36.6 (2010): 1503-1512.
Chicago
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P., Du, Y., Zeng, H., Kang, C."Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells". International Journal of Oncology 36, no. 6 (2010): 1503-1512. https://doi.org/10.3892/ijo_00000637
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Han L, Zhang A, Yang W, Zhou X, Pu P, Du Y, Zeng H and Kang C: Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells. Int J Oncol 36: 1503-1512, 2010.
APA
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P. ... Kang, C. (2010). Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells. International Journal of Oncology, 36, 1503-1512. https://doi.org/10.3892/ijo_00000637
MLA
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P., Du, Y., Zeng, H., Kang, C."Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells". International Journal of Oncology 36.6 (2010): 1503-1512.
Chicago
Zhang, C., Han, L., Zhang, A., Yang, W., Zhou, X., Pu, P., Du, Y., Zeng, H., Kang, C."Global changes of mRNA expression reveals an increased activity of the interferon-induced signal transducer and activator of transcription (STAT) pathway by repression of miR-221/222 in glioblastoma U251 cells". International Journal of Oncology 36, no. 6 (2010): 1503-1512. https://doi.org/10.3892/ijo_00000637
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