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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2010 Volume 37 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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November 2010 Volume 37 Issue 5

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Article

Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53

  • Authors:
    • Shiho Ohnishi
    • Manabu Futamura
    • Hiroki Kamino
    • Yasuyuki Nakamura
    • Noriaki Kitamura
    • Yuji Miyamoto
    • Takafumi Miyamoto
    • Daisuke Shinogi
    • Olga Goda
    • Hirofumi Arakawa
  • View Affiliations / Copyright

    Affiliations: Cancer Medicine and Biophysics Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
  • Pages: 1133-1141
    |
    Published online on: November 1, 2010
       https://doi.org/10.3892/ijo_00000765
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Abstract

NEEP21, also designated D4S234E or NSG1, is an endosomal protein expressed in neuronal cells under normal conditions. Here, we report that NEEP21 is a direct transcriptional target gene of the tumor suppressor p53. NEEP21 expression is inducible in non-neuronal human cancer cell lines by exposure to adriamycin, hydrogen peroxide, UV and γ-ray in a p53-dependent manner. Chromatin immunoprecipitation assay indicated that a potential p53-binding site (p53BS) is located in intron 1 of the NEEP21 gene. A reporter assay confirmed that p53BS has p53-responsive activity. The heterologous luciferase gene containing p53BS is also transactivated by p73-β and p63-γ. The introduction of the NEEP21 gene into various cancer cell lines suppressed cell growth. Infection with an adenovirus vector containing NEEP21 induced apoptotic cell death via caspase-3 activation in many cancer cell lines. The expression of NEEP21 mRNA was remarkably induced by γ-ray irradiation in the spleen of p53+/+ mice but not in that of p53−/− mice. These results suggest that NEEP21 may play a critical role in apoptosis as a mediator of p53.

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Copy and paste a formatted citation
Spandidos Publications style
Ohnishi S, Futamura M, Kamino H, Nakamura Y, Kitamura N, Miyamoto Y, Miyamoto T, Shinogi D, Goda O, Arakawa H, Arakawa H, et al: Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. Int J Oncol 37: 1133-1141, 2010.
APA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y. ... Arakawa, H. (2010). Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. International Journal of Oncology, 37, 1133-1141. https://doi.org/10.3892/ijo_00000765
MLA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37.5 (2010): 1133-1141.
Chicago
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37, no. 5 (2010): 1133-1141. https://doi.org/10.3892/ijo_00000765
Copy and paste a formatted citation
x
Spandidos Publications style
Ohnishi S, Futamura M, Kamino H, Nakamura Y, Kitamura N, Miyamoto Y, Miyamoto T, Shinogi D, Goda O, Arakawa H, Arakawa H, et al: Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. Int J Oncol 37: 1133-1141, 2010.
APA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y. ... Arakawa, H. (2010). Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. International Journal of Oncology, 37, 1133-1141. https://doi.org/10.3892/ijo_00000765
MLA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37.5 (2010): 1133-1141.
Chicago
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37, no. 5 (2010): 1133-1141. https://doi.org/10.3892/ijo_00000765
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