Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372

  • Authors:
    • Wai Kien Yip
    • Sarot Cheenpracha
    • Leng Chee Chang
    • Coy Choke Ho
    • Heng Fong Seow
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000774
  • Pages: 1229-1241
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Abstract

Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fractions from a novel strain, Streptomyces sp. H7372. One of the fractions, designated as 31-2, exhibited the strongest growth-inhibitory effect and thereby was selected for further studies. 31-2 exerted a growth-inhibitory effect on a panel of 15 human cancer and 2 non-malignant cell lines. In MCF-7 and MDA-MB-231 breast cancer cells, 31-2 induced a cytostatic (anti-proliferative) effect without causing cytotoxicity (cell death). Our data suggest that the cytostasis resulted from cell cycle arrest at the G1 phase in MCF-7 cells and at the S phase in MDA-MB-231 cells. Western blot analysis demonstrated a modulation of phosphorylation of the Rb and CDC2 proteins and of CDK4, cyclin D1 and cyclin D3 in the 31-2-treated breast cancer cell lines. The protein levels of CDK2, CDK6, and PCNA were not affected by 31-2 treatment. 31-2 also exhibited an anti-invasive effect in MDA-MB-231 cells. However, this effect is not attributed to the modulation of proteolytic activity in MDA-MB-231 cells as the enzymatic degradation of type IV collagen was not affected by 31-2. The 31-2 is a potent cytostatic and anti-invasive agent and modulates the cell cycle pathway. Together, these results will have important implications in searching for novel approaches to treat cancer.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yip WK, Cheenpracha S, Chang LC, Ho CC and Seow HF: Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. Int J Oncol 37: 1229-1241, 2010
APA
Yip, W.K., Cheenpracha, S., Chang, L.C., Ho, C.C., & Seow, H.F. (2010). Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. International Journal of Oncology, 37, 1229-1241. https://doi.org/10.3892/ijo_00000774
MLA
Yip, W. K., Cheenpracha, S., Chang, L. C., Ho, C. C., Seow, H. F."Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372". International Journal of Oncology 37.5 (2010): 1229-1241.
Chicago
Yip, W. K., Cheenpracha, S., Chang, L. C., Ho, C. C., Seow, H. F."Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372". International Journal of Oncology 37, no. 5 (2010): 1229-1241. https://doi.org/10.3892/ijo_00000774