Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways

Kuo,Jehn-Hwa; Chu,Yung-Lin; Yang,Jai-Sing; Lin,Jing-Pin; Lai,Kuang-Chi; Kuo,Hsiu-Maan; Hsia,Te-Chun; Chung,Jing-Gung

doi:10.3892/ijo_00000775

November 2010 Volume 37 Issue 5

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November 2010 Volume 37 Issue 5

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Article

Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways

Authors:
- Jehn-Hwa Kuo
- Yung-Lin Chu
- Jai-Sing Yang
- Jing-Pin Lin
- Kuang-Chi Lai
- Hsiu-Maan Kuo
- Te-Chun Hsia
- Jing-Gung Chung
View Affiliations / Copyright

Affiliations: Special Class of Healthcare, Industry Management, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C.
Pages: 1243-1250
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Published online on: November 1, 2010

https://doi.org/10.3892/ijo_00000775
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Abstract

Cantharidin has shown potent anticancer activities on many types of human cancer cells. This study was performed to elucidate whether mitochondria and caspases are involved in the modulation of apoptosis and cell cycle arrest by cantharidin in human bladder cancer cells. The effect of cantharidin on cell cycle arrest, apoptosis, caspases, reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry, and the levels of apoptosis-associated proteins and its regulatory molecules were studied by Western blotting. Cantharidin-induced apoptosis and DNA damage was determined by flow cytometric analysis, DAPI staining and Comet assay. After cantharidin treatment, the active forms of caspase-3, -8 and -9 were promoted. Cantharidin-induced apoptosis was associated with enhanced ROS and Ca2+ generations, caused DNA damage, decreased the levels of ΔΨm and promoted Endo G and AIF released from mitochondria. Cantharidin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of cyclin E and Cdc25c but promoted the levels of p21 and p-p53. Cantharidin-induced apoptosis was accompanied with up-regulation of the protein expression of Bax and PARP, but down-regulation of the protein levels of Bcl-2, resulting in dysfunction of mitochondria then led to Endo G and AIF release for causing induction of apoptosis.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways

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Abstract

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