Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells

  • Authors:
    • Jiumao Lin
    • Youqin Chen
    • Lihui Wei
    • Xuzhen Chen
    • Wei Xu
    • Zhenfeng Hong
    • Thomas J. Sferra
    • Jun Peng
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000785
  • Pages: 1331-1338
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Abstract

Hedyotis Diffusa Willd has been used as a major component in several Chinese medicine formulations for the clinical treatment of colorectal cancer. However, the molecular mechanism of the anti-cancer activity of Hedyotis Diffusa Willd remains unclear. In the present study, we investigated the cellular effects of the ethanol extract of Hedyotis Diffusa Willd (EEHDW) in the HT-29 human colon carcinoma cell line. We found that EEHDW inhibited the growth of HT-29 cells demonstrating EEHDW-induced cell morphological changes and reduced cell viability in a dose- and time-dependent manner. Furthermore, we observed that EEHDW treatment resulted in DNA fragmentation, loss of plasma membrane asymmetry, collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, and increase of the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2, suggesting that the HT-29 cell growth inhibitory activity of EEHDW was due to mitochondrion-mediated apoptosis, which may partly explain the anti-cancer activity of Hedyotis Diffusa Willd.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lin J, Chen Y, Wei L, Chen X, Xu W, Hong Z, Sferra TJ and Peng J: Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells. Int J Oncol 37: 1331-1338, 2010.
APA
Lin, J., Chen, Y., Wei, L., Chen, X., Xu, W., Hong, Z. ... Peng, J. (2010). Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells. International Journal of Oncology, 37, 1331-1338. https://doi.org/10.3892/ijo_00000785
MLA
Lin, J., Chen, Y., Wei, L., Chen, X., Xu, W., Hong, Z., Sferra, T. J., Peng, J."Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells". International Journal of Oncology 37.5 (2010): 1331-1338.
Chicago
Lin, J., Chen, Y., Wei, L., Chen, X., Xu, W., Hong, Z., Sferra, T. J., Peng, J."Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells". International Journal of Oncology 37, no. 5 (2010): 1331-1338. https://doi.org/10.3892/ijo_00000785