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Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines

  • Authors:
    • Masahito Hagio
    • Yoko Matsuda
    • Taeko Suzuki
    • Toshiyuki Ishiwata
  • View Affiliations / Copyright

    Affiliations: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo 113-8602, Japan
  • Pages: 83-87
    |
    Published online on: June 8, 2012
       https://doi.org/10.3892/mco.2012.1
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Abstract

Nestin, a class VI intermediate filament, is a neuronal stem/progenitor cell marker that is also expressed by various types of cancer, including pancreatic ductal adenocarcinoma (PDAC). We previously detected nestin expression in approximately 30% of PDAC cases, and found that nestin promotes the migration, invasion and metastasis of cells. Findings of recent studies have shown that epithelial mesenchymal transition (EMT) is important in the invasion and migration of cancer. In the present study, we investigated whether an altered nestin expression affected the expression levels of EMT markers in PDAC cells. Two human PDAC cell lines, PK-45H and KLM-1, in which nestin was suppressed and overexpressed, respectively, were used. The expression levels of the EMT-related molecules E-cadherin, Snail, Slug and Twist were analyzed using quantitative RT-PCR. Results showed that E-cadherin expression was decreased in nestin-overexpressed KLM-1 cells, and increased in nestin-suppressed PK-45H cells. Snail gene expression in the PDAC cells was altered concomitantly with the changes in nestin expression, while the Slug gene expression was significantly decreased in nestin-overexpressed KLM-1 cells. The Twist gene expression was below the detection limit in the two PDAC cell lines. The present findings indicated that nestin may be involved in the control of cancer behaviors in PDAC via the modulation of EMT-related molecules.
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1. 

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T and Thun MJ: Cancer statistics, 2008. CA Cancer J Clin. 58:71–96. 2008.

2. 

Lendahl U, Zimmerman LB and McKay RD: CNS stem cells express a new class of intermediate filament protein. Cell. 60:585–595. 1990.

3. 

Esni F, Stoffers DA, Takeuchi T and Leach SD: Origin of exocrine pancreatic cells from nestin-positive precursors in developing mouse pancreas. Mech Dev. 121:15–25. 2004.

4. 

Delacour A, Nepote V, Trumpp A and Herrera PL: Nestin expression in pancreatic exocrine cell lineages. Mech Dev. 121:3–14. 2004.

5. 

Ishiwata T, Kudo M, Onda M, Fujii T, Teduka K, Suzuki T, Korc M and Naito Z: Defined localization of nestin-expressing cells in L-arginine-induced acute pancreatitis. Pancreas. 32:360–368. 2006.

6. 

Parry S, Savage K, Marchio C and Reis-Filho JS: Nestin is expressed in basal-like and triple negative breast cancers. J Clin Pathol. 61:1045–1050. 2008.

7. 

Carrière C, Seeley ES, Goetze T, Longnecker DS and Korc M: The Nestin progenitor lineage is the compartment of origin for pancreatic intraepithelial neoplasia. Proc Natl Acad Sci USA. 104:4437–4442. 2007.

8. 

Matsuda Y, Naito Z, Kawahara K, Nakazawa N, Korc M and Ishiwata T: Nestin is a novel target for suppressing pancreatic cancer cell migration, invasion and metastasis. Cancer Biol Ther. 11:512–523. 2011.

9. 

Micalizzi DS, Farabaugh SM and Ford HL: Epithelialmesenchymal transition in cancer: parallels between normal development and tumor progression. J Mammary Gland Biol Neoplasia. 15:117–134. 2010.

10. 

Larue L and Bellacosa A: Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 39 kinase/AKT pathways. Oncogene. 24:7443–7454. 2005.

11. 

Hay ED: The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it. Dev Dyn. 233:706–720. 2005.

12. 

Casas E, Kim J, Bendesky A, Ohno-Machado L, Wolfe CJ and Yang J: Snail2 is an essential mediator of Twist1-induced epithelial mesenchymal transition and metastasis. Cancer Res. 71:245–254. 2011.

13. 

Hotz B, Arndt M, Dullat S, Bhargava S, Buhr HJ and Hotz HG: Epithelial to mesenchymal transition: expression of the regulators snail, slug, and twist in pancreatic cancer. Clin Cancer Res. 13:4769–4776. 2007.

14. 

Liu J and Brown RE: Immunohistochemical detection of epithelialmesenchymal transition associated with stemness phenotype in anaplastic thyroid carcinoma. Int J Clin Exp Pathol. 3:755–762. 2010.

15. 

Hay ED: An overview of epithelio-mesenchymal transformation. Acta Anat (Basel). 154:8–20. 1995.

16. 

Birchmeier C, Birchmeier W and Brand-Saberi B: Epithelialmesenchymal transitions in cancer progression. Acta Anat (Basel). 156:217–226. 1996.

17. 

Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2:442–454. 2002.

18. 

Kalluri R and Neilson EG: Epithelial-mesenchymal transition and its implications for fibrosis. J Clin Invest. 112:1776–1784. 2003.

19. 

Taylor MA, Parvani JG and Schiemann WP: The pathophysiology of epithelial-mesenchymal transition induced by transforming growth factor-beta in normal and malignant mammary epithelial cells. J Mammary Gland Biol Neoplasia. 15:169–190. 2010.

20. 

Savagner P: Leaving the neighborhood: molecular mechanisms involved during epithelial-mesenchymal transition. Bioessays. 23:912–923. 2001.

21. 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005.

22. 

Schaeffer DF, Assi K, Chan K, Buczkowski AK, Chung SW, Scudamore CH, Weiss A, Salh B and Owen DA: Tumor expression of integrin-linked kinase (ILK) correlates with the expression of the E-cadherin repressor snail: an immunohistochemical study in ductal pancreatic adenocarcinoma. Virchows Arch. 456:261–268. 2010.

23. 

Nieto MA, Sargent MG, Wilkinson DG and Cooke J: Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science. 264:835–839. 1994.

24. 

Shields MA, Krantz SB, Bentrem DJ, Dangi-Garimella S and Munshi HG: Interplay between β1-integrin and Rho signaling regulates differential scattering and motility of pancreatic cancer cells by snail and Slug proteins. J Biol Chem. 287:6218–6229. 2012.

25. 

Montserrat N, Gallardo A, Escuin D, Catasus L, Prat J, Gutiérrez-Avignó FJ, Peiró G, Barnadas A and Lerma E: Repression of E-cadherin by SNAIL, ZEB1, and TWIST in invasive ductal carcinomas of the breast: a cooperative effort? Hum Pathol. 42:103–110. 2011.

26. 

Cates JM, Byrd RH, Fohn LE, Tatsas AD, Washington MK and Black CC: Epithelial-mesenchymal transition markers in pancreatic ductal adenocarcinoma. Pancreas. 38:e1–e6. 2009.

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Copy and paste a formatted citation
Spandidos Publications style
Hagio M, Matsuda Y, Suzuki T and Ishiwata T: Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines. Mol Clin Oncol 1: 83-87, 2013.
APA
Hagio, M., Matsuda, Y., Suzuki, T., & Ishiwata, T. (2013). Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines. Molecular and Clinical Oncology, 1, 83-87. https://doi.org/10.3892/mco.2012.1
MLA
Hagio, M., Matsuda, Y., Suzuki, T., Ishiwata, T."Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines". Molecular and Clinical Oncology 1.1 (2013): 83-87.
Chicago
Hagio, M., Matsuda, Y., Suzuki, T., Ishiwata, T."Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines". Molecular and Clinical Oncology 1, no. 1 (2013): 83-87. https://doi.org/10.3892/mco.2012.1
Copy and paste a formatted citation
x
Spandidos Publications style
Hagio M, Matsuda Y, Suzuki T and Ishiwata T: Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines. Mol Clin Oncol 1: 83-87, 2013.
APA
Hagio, M., Matsuda, Y., Suzuki, T., & Ishiwata, T. (2013). Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines. Molecular and Clinical Oncology, 1, 83-87. https://doi.org/10.3892/mco.2012.1
MLA
Hagio, M., Matsuda, Y., Suzuki, T., Ishiwata, T."Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines". Molecular and Clinical Oncology 1.1 (2013): 83-87.
Chicago
Hagio, M., Matsuda, Y., Suzuki, T., Ishiwata, T."Nestin regulates epithelial-mesenchymal transition marker expression in pancreatic ductal adenocarcinoma cell lines". Molecular and Clinical Oncology 1, no. 1 (2013): 83-87. https://doi.org/10.3892/mco.2012.1
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