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Article

Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer

  • Authors:
    • Katsunori Tozuka
    • Jun Horiguchi
    • Daisuke Takata
    • Nana Rokutanda
    • Rin Nagaoka
    • Hideaki Tokiniwa
    • Mami Kikuchi
    • Ayako Satou
    • Hiroyuki Takei
    • Izumi Takeyoshi
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan, Division of Breast Surgery, Saitama Cancer Center, Kitaadachi, Saitama 362-0806, Japan
  • Pages: 93-99
    |
    Published online on: July 26, 2012
       https://doi.org/10.3892/mco.2012.4
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Abstract

Anthracyclines and taxanes are standard anticancer drugs used in breast cancer chemotherapy. In general, the efficacy of chemotherapy is lower in patients with estrogen receptor (ER)-positive tumors compared to patients with ER-negative tumors. Although less chemosensitive, ER-positive disease includes a subset of patients who significantly benefit from adjuvant chemotherapy. The collagen gel droplet-embedded culture-drug sensitivity test (CD-DST) is an in vitro chemosensitivity test that has several advantages over conventional tests. The aim of the present study was to examine the correlation between CD-DST and the expression of Ki67, an indicator of tumor proliferation, to evaluate the efficacy of anthracyclines and taxanes in patients with ER-positive and human epidermal growth factor receptor 2 (HER2)-negative breast cancer. CD-DST was performed in 68 patients with ER-positive and HER2-negative breast cancer between August 2001 and November 2006. The specimens obtained during surgery were used for the CD-DST and immunohistological examination of Ki67 expression. Chemosensitivity to the anticancer drugs adriamycin (ADM), epirubicin (EPI), docetaxel (DOC) and paclitaxel (PTX) was estimated using CD-DST. Results obtained from the CD-DST showed the chemosensitivity to each anticancer drug to be ADM, 20.4%; EPI, 67.3%; DOC, 66.7% and PTX, 43.6%. Ki67 expression was significantly higher in the group that was sensitive to DOC compared to the group that was resistant to DOC (P=0.021) and PTX (P=0.033). In addition, a significant correlation was observed between a Ki67 labeling index (LI) of >30% and chemosensitivity to PTX. In conclusion, results obtained from CD-DST and Ki67 expression levels are able to identify a subset of patients with ER-positive and HER2-negative breast cancer who exhibit sensitivity to chemotherapy, particularly to taxane therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Tozuka K, Horiguchi J, Takata D, Rokutanda N, Nagaoka R, Tokiniwa H, Kikuchi M, Satou A, Takei H, Takeyoshi I, Takeyoshi I, et al: Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer. Mol Clin Oncol 1: 93-99, 2013.
APA
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H. ... Takeyoshi, I. (2013). Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer. Molecular and Clinical Oncology, 1, 93-99. https://doi.org/10.3892/mco.2012.4
MLA
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H., Kikuchi, M., Satou, A., Takei, H., Takeyoshi, I."Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer". Molecular and Clinical Oncology 1.1 (2013): 93-99.
Chicago
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H., Kikuchi, M., Satou, A., Takei, H., Takeyoshi, I."Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer". Molecular and Clinical Oncology 1, no. 1 (2013): 93-99. https://doi.org/10.3892/mco.2012.4
Copy and paste a formatted citation
x
Spandidos Publications style
Tozuka K, Horiguchi J, Takata D, Rokutanda N, Nagaoka R, Tokiniwa H, Kikuchi M, Satou A, Takei H, Takeyoshi I, Takeyoshi I, et al: Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer. Mol Clin Oncol 1: 93-99, 2013.
APA
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H. ... Takeyoshi, I. (2013). Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer. Molecular and Clinical Oncology, 1, 93-99. https://doi.org/10.3892/mco.2012.4
MLA
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H., Kikuchi, M., Satou, A., Takei, H., Takeyoshi, I."Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer". Molecular and Clinical Oncology 1.1 (2013): 93-99.
Chicago
Tozuka, K., Horiguchi, J., Takata, D., Rokutanda, N., Nagaoka, R., Tokiniwa, H., Kikuchi, M., Satou, A., Takei, H., Takeyoshi, I."Collagen gel droplet-embedded culture-drug sensitivity test and Ki67 expression in estrogen receptor-positive and HER2-negative breast cancer". Molecular and Clinical Oncology 1, no. 1 (2013): 93-99. https://doi.org/10.3892/mco.2012.4
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