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Molecular and Clinical Oncology
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells

  • Authors:
    • Wu‑Qing Wang
    • Jin‑Feng Wu
    • Xiao‑Qing Xiao
    • Qin Xiao
    • Jing Wang
    • Fu‑Guo Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The Central Hospital of Minhang, Shanghai 201199, P.R. China, Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Dermatology, East Hospital, School of Medicine, Tongji University, Shanghai 200120, P.R. China
  • Pages: 858-862
    |
    Published online on: July 11, 2013
       https://doi.org/10.3892/mco.2013.145
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Abstract

Melanocytes are found scattered throughout the basal layer of the epidermis. Following hormone or ultraviolet (UV) light stimulation, the melanin pigments contained in melanocytes are transferred through the dendrites to the surrounding keratinocytes to protect against UV light damage or carcinogenesis. This has been considered as a morphological indicator of melanocytes and melanoma cells. Small GTPases of the Rho family have been implicated in the regulation of actin reorganization underlying dendrite formation in melanocytes and melanoma cells. It has been proven that ultraviolet light plays a pivotal role in melanocyte dendrite formation; however, the molecular mechanism underlying this process has not been fully elucidated. the effect of small GTPases, such as Rac1 and RhoA, on the morphology of B16 melanoma cells treated with narrow‑band UVB radiation was investigated. The morphological changes were observed under a phase contrast microscope and the F‑actin microfilament of the cytoskeleton was observed under a laser scanning confocal microscope. The pull‑down assay was performed to detect the activity of the small GTPases Rac1 and RhoA. The morphological changes were evident, with globular cell bodies and increased numbers of tree branch‑like dendrites. the cytoskeletal F‑actin appeared disassembled following narrow‑band UVB irradiation of B16 melanoma cells. Treatment of B16 melanoma cells with narrow‑band UVB radiation resulted in the activation of Rac1 in a time‑dependent manner. In conclusion, the present study may provide a novel method through which narrow‑band UVB radiation may be used to promote dendrite formation by activating the Rac1 signaling pathway, resulting in F‑actin rearrangement in B16 melanoma cells.
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1 

Chakraborty AK, Funasaka Y, Araki K, Horikawa T and Ichihashi M: Evidence that the small GTPase Rab8 is involved in melanosome traffic and dendrite extension in B16 melanoma cells. Cell Tissue Res. 314:381–388. 2003. View Article : Google Scholar

2 

Fitzpatrick B, Miyamoto M and Ishikawa K: The evolution of concepts of melanin biology. Arch Dermatol. 96:305–323. 1967. View Article : Google Scholar : PubMed/NCBI

3 

Scott G: Rac and Rho: the story behind melanocyte dendrite formation. Pigment Cell Res. 15:322–330. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Hara M, Yaar M and Gilchrest BA: Endothelin-1 of keratinocyte origin is a mediator of melanocyte dendricity. J Invest Dermatol. 105:744–748. 1995. View Article : Google Scholar : PubMed/NCBI

5 

Busca R, Bertolotto C, Abbe P, Engalo W, Ishizaki T, Narumiya S, Boquet P, Ortonne JP and Ballotti R: Inhibition of Rho is required for cAMP-induced melanoma cell differentiation. Mol Biol Cell. 9:1367–1378. 1998. View Article : Google Scholar : PubMed/NCBI

6 

Yoshida M, Takahashi Y and Inoue S: Histamine induces melanogenesis and morphologic changes by protein kinase A activation via H2 receptors in human normal melanocytes. J Invest Dermatol. 114:334–342. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Hata K, Hori K, Murata J and Takahashi S: Remodeling of actin cytoskeleton in lupeol-induced B16 2F2 cell differentiation. J Biochem. 138:467–472. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Nowak JM, Grzanka A, Zuryń A and Stepień A: The Rho protein family and its role in the cellular cytoskeleton. Postepy Hig Med Dosw (Online). 62:110–117. 2008.(In Polish).

9 

Parri M and Chiarugi P: Rac and Rho GTPases in cancer cell motility control. Cell Commun Signal. 8:232010. View Article : Google Scholar : PubMed/NCBI

10 

Haass NK, Smalley KS, Li L and Herlyn M: Adhesion, migration and communication in melanocytes and melanoma. Pigment Cell Res. 18:150–159. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Ongusaha PP, Kim HG, Boswell SA, Ridley AJ, Der CJ, Dotto GP, Kim YB, Aaronson SA and Lee SW: RhoE is a pro-survival p53 target gene that inhibits ROCK I-mediated apoptosis in response to genotoxic stress. Curr Biol. 16:2466–2472. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Benitah SA, Valerón PF and Lacal JC: ROCK and nuclear factor-κB-dependent activation of cyclooxygenase-2 by Rho GTPases: effects on tumor growth and therapeutic consequences. Mol Biol Cell. 14:3041–3054. 2003.

13 

Etienne-Manneville S and Hall A: Rho GTPases in cell biology. Nature. 420:629–635. 2002. View Article : Google Scholar

14 

Burridge K and Wennerberg K: Rho and Rac take center stage. Cell. 116:167–179. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Kim MY, Choi TY, Kim JH, Lee JH, Kim JG, Sohn KC, Yoon KS, Kim CD, Lee JH and Yoon TJ: MKK6 increases the melanocyte dendricity through the regulation of Rho family GTPases. J Dermatol Sci. 60:114–119. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Ito Y, Kanamaru A and Tada A: Centaureidin promotes dendrite retraction of melanocytes by activating Rho. Biochim Biophys Acta. 1760:487–494. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Scott G and Leopardi S: The cAMP signaling pathway has opposing effects on Rac and Rho in B16F10 cells: implications for dendrite formation in melanocytic cells. Pigment Cell Res. 16:139–148. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Jimbow M and Jimbow K: Pigmentary disorders in oriental skin. Clin Dermatol. 7:11–27. 1989. View Article : Google Scholar : PubMed/NCBI

19 

Pichardo R, Vallejos Q, Feldman SR, Schulz MR, Verma A, Quandt SA and Arcury TA: The prevalence of melasma and its association with quality of life in adult male Latino migrant workers. Int J Dermatol. 48:22–26. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Kang WH, Yoon KH, Lee ES, Kim J, Lee KB, Yim H, Sohn S and Im S: Melasma: histopathological characteristics in 56 Korean patients. Br J Dermatol. 146:228–237. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Hall A: Rho GTPases and the control of cell behaviour. Biochem Soc Trans. 33(Pt 5): 891–895. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Scott GA and Cassidy L: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model. J Invest Dermatol. 111:243–250. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Katoh H, Aoki J, Ichikawa A and Negishi M: p160 RhoA-binding kinase induces neurite retraction. J Biol Chem. 273:2489–2492. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Sander EE, ten Klooster JP, van Delft S, van der Kammen RA and Collard JG: Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J Cell Biol. 147:1009–1022. 1999. View Article : Google Scholar

25 

Ridley AJ, Paterson HF, Johnston CL, Diekmann D and Hall A: The small GTP-binding protein Rac regulates growth factor-induced membrane ruffling. Cell. 70:401–410. 1992. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang WQ, Wu JF, Xiao XQ, Xiao Q, Wang J and Zuo FG: Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells. Mol Clin Oncol 1: 858-862, 2013.
APA
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., & Zuo, F. (2013). Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells. Molecular and Clinical Oncology, 1, 858-862. https://doi.org/10.3892/mco.2013.145
MLA
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., Zuo, F."Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells". Molecular and Clinical Oncology 1.5 (2013): 858-862.
Chicago
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., Zuo, F."Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells". Molecular and Clinical Oncology 1, no. 5 (2013): 858-862. https://doi.org/10.3892/mco.2013.145
Copy and paste a formatted citation
x
Spandidos Publications style
Wang WQ, Wu JF, Xiao XQ, Xiao Q, Wang J and Zuo FG: Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells. Mol Clin Oncol 1: 858-862, 2013.
APA
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., & Zuo, F. (2013). Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells. Molecular and Clinical Oncology, 1, 858-862. https://doi.org/10.3892/mco.2013.145
MLA
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., Zuo, F."Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells". Molecular and Clinical Oncology 1.5 (2013): 858-862.
Chicago
Wang, W., Wu, J., Xiao, X., Xiao, Q., Wang, J., Zuo, F."Narrow‑band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells". Molecular and Clinical Oncology 1, no. 5 (2013): 858-862. https://doi.org/10.3892/mco.2013.145
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