Radiation effects on DNA content of cervical cancer cells: A rapid evaluation of radiation sensitivity by laser scanning cytometry

Fujiyoshi,Naoki; Ushijima,Kimio; Kawano,Kouichiro; Fujiyoshi,Keizo; Yamaguchi,Tomohiko; Araki,Yuko; Kakuma,Tatsuyuki; Watanabe,Sumiko; Kaku,Tsunehisa; Nishida,Takashi; Kamura,Toshiharu

doi:10.3892/mco.2014.413

January-February 2015 Volume 3 Issue 1

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January-February 2015 Volume 3 Issue 1

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Article

Radiation effects on DNA content of cervical cancer cells: A rapid evaluation of radiation sensitivity by laser scanning cytometry

Authors:
- Naoki Fujiyoshi
- Kimio Ushijima
- Kouichiro Kawano
- Keizo Fujiyoshi
- Tomohiko Yamaguchi
- Yuko Araki
- Tatsuyuki Kakuma
- Sumiko Watanabe
- Tsunehisa Kaku
- Takashi Nishida
- Toshiharu Kamura
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, School of Medicine, Kurume University, Kurume, Fukuoka, Japan, Department of Diagnostic Pathology, Kurume University Hospital, Kurume University, Kurume, Japan, Department of Biostatistics, School of Medicine, Kurume University, Kurume, Japan, Department of Health Science, Faculty of Medicine, Kyushu University, Higashi‑ku, Fukuoka, Japan, Department of Gynecology, Oita Prefecture Saiseikai Hita Hospital, Hita, Oita, Japan
Pages: 51-54
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Published online on: September 9, 2014

https://doi.org/10.3892/mco.2014.413
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Abstract

Since uterine cervical cancer is regarded as a radiosensive tumor, ionizing radiation is the most frequently used treatment modality against the disease. Although the crucial end‑point is radiation‑induced cell death, the tumors are not equally sensitive to radiation. Determining the criteria that may be used to predict tumor radiosensitivity is of importance; however, little success has been achieved thus far. In radioresistant cases the therapeutic strategy should be changed, thereby avoiding ineffective or unnecessary treatment. Furthermore, identification of the underlying molecular processes leading to radioresistance may lead to novel radiosensitising strategies. Cervical smears were obtained from seven patients with locally advanced cervical cancer following each radiotherapy, and the radiation‑induced damage of cancer tissue was examined by routine cytology. Since the formation of DNA double‑strand breaks is considered critical for the cytocidal effect of radiation therapy, the molecular changes of the neoplastic cells were also assessed by laser scanning cytometry (LSC). Radiation‑induced morphological changes of cancer cells were evident at a dose of 7.2 Gy, whereas increased DNA content (or DNA index) was observed prior to the onset of morphological changes. Molecular change was detected earlier than the morphological change of the irradiated cancer cells, indicating the feasibility of LSC in predicting the radiosensitivity of cervical cancer tissue.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Radiation effects on DNA content of cervical cancer cells: A rapid evaluation of radiation sensitivity by laser scanning cytometry

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