Analysis of mitochondrial DNA in Tibetan gastric cancer patients at high altitude

Jiang,Jun; Zhao,Jun‑Hui; Wang,Xue‑Lian; Di,Ji; Liu,Zhi‑Bo; Li,Guo‑Yuan; Wang,Miao‑Zhou; Li,Yan; Chen,Rong; Ge,Ri‑Li

doi:10.3892/mco.2015.539

July-2015 Volume 3 Issue 4

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July-2015 Volume 3 Issue 4

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Article

Analysis of mitochondrial DNA in Tibetan gastric cancer patients at high altitude

Authors:
- Jun Jiang
- Jun‑Hui Zhao
- Xue‑Lian Wang
- Ji Di
- Zhi‑Bo Liu
- Guo‑Yuan Li
- Miao‑Zhou Wang
- Yan Li
- Rong Chen
- Ri‑Li Ge
View Affiliations / Copyright

Affiliations: Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China, Department of Oncology, Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R. China, Department of Surgery, Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R. China
Pages: 875-879
|
Published online on: April 3, 2015

https://doi.org/10.3892/mco.2015.539
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Abstract

The highest risk areas of gastric cancer are currently Japan, Korea and China; Qinghai, a high‑altitude area, has one of the highest gastric cancer rates in China. The incidence of gastric cancer is higher in the Tibetan ethnic group compared to that in the Han ethnic group in Qinghai. This study was conducted to determine the clinical characteristics of mitochondrial DNA (mtDNA) mutations and copy numbers among Tibetans with gastric cancer residing at high altitudes and investigate the association between adaptations to hypoxic conditions and oncogenesis. A total of 23 Tibetan gastric cancer patients and 40 matched controls were recruited in this study. Leukocyte mtDNA genes and copy numbers were analyzed. The haplogroups were classified based on mitochondrial gene sequences. A total of 56.5% of the study participants had used alcohol at some point in their lives and 73.9% were positive for Helicobacter pylori (H. pylori). Eight mutations in 8 mitochondrial genes were identified in 43.4% of the Tibetan cancer patient group. There were no significant differences in leukocyte mtDNA copy number levels based on smoking status, alchohol consumption, obesity or H. pylori infection between the control and cancer groups. Statistical differences were also not found between gastric cancer patients with and those without mtDNA mutations. The majority of Tibetan patients with gastric cancer belonged to the mitochondrial haplogroup M9. In conclusion, Tibetans with gastric cancer residing at high altitudes exhibited a wide spectrum of mtDNA mutations. However, leukocyte mtDNA copy numbers in stage II gastric cancer were not statistically different compared to those in healthy Tibetans.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of mitochondrial DNA in Tibetan gastric cancer patients at high altitude

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Abstract

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