Plasma protoporphyrin IX following administration of 5-aminolevulinic acid as a potential tumor marker

Ota,Urara; Fukuhara,Hideo; Ishizuka,Masahiro; Abe,Fuminori; Kawada,Chiaki; Tamura,Kenji; Tanaka,Tohru; Inoue,Keiji; Ogura,Shun‑Ichiro; Shuin,Taro

doi:10.3892/mco.2015.549

July-2015 Volume 3 Issue 4

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July-2015 Volume 3 Issue 4

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Article

Plasma protoporphyrin IX following administration of 5-aminolevulinic acid as a potential tumor marker

Authors:
- Urara Ota
- Hideo Fukuhara
- Masahiro Ishizuka
- Fuminori Abe
- Chiaki Kawada
- Kenji Tamura
- Tohru Tanaka
- Keiji Inoue
- Shun‑Ichiro Ogura
- Taro Shuin
View Affiliations / Copyright

Affiliations: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1‑6‑1, Tokyo 106‑6020, Japan, Department of Urology, Kochi Medical School, Nankoku, Kochi 783‑8505, Japan, Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226‑8501, Japan
Pages: 797-801
|
Published online on: April 24, 2015

https://doi.org/10.3892/mco.2015.549
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Abstract

Exogenously administered 5-aminolevulinic acid (ALA) is metabolized to protoporphyrin IX (PpIX), which specifically accumulates in cancer cells and emits red fluorescence by blue light irradiation. These phenomena are applied for the intraoperative diagnosis of cancer. Based on the fact that accumulated PpIX in cancer cells is exported extracellularly via the ATP‑binding cassette transporter G2, we hypothesized that the measurement of plasma PpIX concentrations could be applied as a tumor marker for cancer screening. In the present study, the use of plasma samples from bladder cancer patients were evaluated as a tumor marker. ALA, 1.0 g, was orally administered to bladder cancer patients and healthy adults. The plasma concentration of PpIX was measured using a high‑performance liquid chromatography system. The plasma PpIX concentration following ALA administration was significantly higher in bladder cancer patients than that in the healthy adults, suggesting the effectiveness of plasma PpIX analysis following ALA administration for cancer screening. Additionally, 4 h after ALA administration, plasma PpIX showed high sensitivity (94.4%) and high specificity (80.0%).

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Plasma protoporphyrin IX following administration of 5-aminolevulinic acid as a potential tumor marker

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