Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors

Lin,Lien‑Fu; Jin,Jong‑Shiaw; Chen,Jui‑Chang; Huang,Chia‑Chi; Sheu,Jeng‑Horng; Chen,Wenlung; Tsao,Tang‑Yi; Hsu,Chih‑Wei

doi:10.3892/mco.2016.835

Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors

Authors:
- Lien‑Fu Lin
- Jong‑Shiaw Jin
- Jui‑Chang Chen
- Chia‑Chi Huang
- Jeng‑Horng Sheu
- Wenlung Chen
- Tang‑Yi Tsao
- Chih‑Wei Hsu
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Affiliations: Division of Gastroenterology, Department of Internal Medicine, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan R.O.C., Department of Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan R.O.C., Department of Applied Chemistry, National Chia-Yi University, Chia‑Yi 600, Taiwan R.O.C., Center of Nano Bio‑Detection, National Chung Cheng University Chia‑Yi, Chia‑Yi 621, Taiwan R.O.C.
Published online on: March 30, 2016 https://doi.org/10.3892/mco.2016.835
Pages: 971-975

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Abstract

Positive transcriptional elongation factor b (P‑TEFb) contains the catalytic subunit cyclin‑dependent kinase 9 (Cdk9) and the regulatory subunit cyclin T. Cyclin T1 and Cdk9 are the key factors of the PTEFb pathways and are overexpressed in the human head and neck carcinoma cell line. However, there have been limited studies regarding the role of cyclin T1 and Cdk9 in gastric gastrointestinal stromal tumors (GISTs). The aim of the present study was to assess the association between cyclin T1 and Cdk9 and their clinical significance in gastric GISTs. A total of 30 gastric GIST patients who underwent either laparoscopic or laparotomic partial gastrectomy were enrolled in the study. The surgical tissue slides were stained with Cdk9 and cyclin T1 antibodies, and the immunohistochemistry scores and disease‑free survival (DFS) were analyzed. Ten patients were cyclin T1‑positive, and 20 were negative. All 11 patients with recurrent tumors or distant metastases were cyclin T1‑negative patients. Old age, large tumor size, a high Ki67 IHC staining score, high mitotic count and negative cyclin T1 staining revealed a worse clinical outcome in univariate analysis. By contrast, the Cdk9 score was not associated with clinical parameters. The Kaplan‑Meier survival curve illustrated that the DFS rate of the patients with negative cyclin T1 staining was significantly lower than that of the patients with positive cyclin T1 staining. Positive expression of cyclin T1 was a good prognostic factor in patients with gastric GISTs.

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