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Article

Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients

  • Authors:
    • Nikolaos Koufos
    • John Syrios
    • Despina Michailidou
    • Ioannis D. Xynos
    • Andreas Lazaris
    • Nicolaos Kavantzas
    • Periclis Tomos
    • Stamatis Kakaris
    • Christos Kosmas
    • Nikolas Tsavaris
  • View Affiliations / Copyright

    Affiliations: Oncology Unit, Department of Pathophysiology, Laiko General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece, 2nd Department of Medical Oncology, ‘St. Savvas’ Cancer Hospital, 11522 Athens, Greece, Imperial Clinical Trials Unit‑Cancer, Department of Surgery and Cancer, Charing Cross Hospital, Imperial College, London W6 8RF, UK, 1st Department of Pathology, Laiko General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece, 2nd Department of Propedeutic Surgery, Laiko General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece, 1st Department of Thoracic Surgery, ‘Sotiria’ General Hospital of Chest Diseases, 11527 Athens, Greece, 2nd Division of Medical Oncology, Department of Medicine, Metaxa Cancer Hospital, 18537 Piraeus, Greece
  • Pages: 440-446
    |
    Published online on: July 27, 2016
       https://doi.org/10.3892/mco.2016.966
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Abstract

The expression of various angiogenic factors was assessed in tumour samples of patients with stage III non-small-cell lung cancer (NSCLC) and further evaluated in terms of response to induction paclitaxel‑ifosfamide‑cisplatin chemotherapy. Freshly isolated lung tumour specimens obtained by bronchoscopy from 70 stage IIIA NSCLC chemotherapy‑naïve patients were sampled and analysed for vascular endothelial growth factor receptor (VEGFR)‑1, VEGFR‑2 and VEGFR‑3. Microvessel density was assessed through evaluating the angiogenic markers CD34 and CD105. Immunostaining scores were calculated by multiplying the percentage of labeled cells by the intensity of staining for each examined parameter. The overall mean immunostaining score value from all NSCLC samples was 7.83, 5.56 and 15.86 for VEGFR‑1, VEGFR‑2 and VEGFR‑3, respectively. The overall mean value of the endothelial antigen CD34 was 16.29, whereas the expression of the CD105 antigen in endothelial cells yielded a multivariate distribution. Patients who responded to chemotherapy expressed significantly higher VEGFR‑1 and VEGFR‑3 mean values compared with non‑responders (P<0.001). No significant difference was noted in VEGFR‑2 mean values between these two groups (P=0.06). The CD34 mean value was significantly higher in responders (P<0.001), whereas there was no significant difference in CD105 expression between the two groups (P=0.07). Angiogenic marker expression proved to be a potential predictive factor of response to chemotherapy in stage III NSCLC. which merits further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Koufos N, Syrios J, Michailidou D, Xynos ID, Lazaris A, Kavantzas N, Tomos P, Kakaris S, Kosmas C, Tsavaris N, Tsavaris N, et al: Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients. Mol Clin Oncol 5: 440-446, 2016.
APA
Koufos, N., Syrios, J., Michailidou, D., Xynos, I.D., Lazaris, A., Kavantzas, N. ... Tsavaris, N. (2016). Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients. Molecular and Clinical Oncology, 5, 440-446. https://doi.org/10.3892/mco.2016.966
MLA
Koufos, N., Syrios, J., Michailidou, D., Xynos, I. D., Lazaris, A., Kavantzas, N., Tomos, P., Kakaris, S., Kosmas, C., Tsavaris, N."Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients". Molecular and Clinical Oncology 5.4 (2016): 440-446.
Chicago
Koufos, N., Syrios, J., Michailidou, D., Xynos, I. D., Lazaris, A., Kavantzas, N., Tomos, P., Kakaris, S., Kosmas, C., Tsavaris, N."Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients". Molecular and Clinical Oncology 5, no. 4 (2016): 440-446. https://doi.org/10.3892/mco.2016.966
Copy and paste a formatted citation
x
Spandidos Publications style
Koufos N, Syrios J, Michailidou D, Xynos ID, Lazaris A, Kavantzas N, Tomos P, Kakaris S, Kosmas C, Tsavaris N, Tsavaris N, et al: Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients. Mol Clin Oncol 5: 440-446, 2016.
APA
Koufos, N., Syrios, J., Michailidou, D., Xynos, I.D., Lazaris, A., Kavantzas, N. ... Tsavaris, N. (2016). Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients. Molecular and Clinical Oncology, 5, 440-446. https://doi.org/10.3892/mco.2016.966
MLA
Koufos, N., Syrios, J., Michailidou, D., Xynos, I. D., Lazaris, A., Kavantzas, N., Tomos, P., Kakaris, S., Kosmas, C., Tsavaris, N."Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients". Molecular and Clinical Oncology 5.4 (2016): 440-446.
Chicago
Koufos, N., Syrios, J., Michailidou, D., Xynos, I. D., Lazaris, A., Kavantzas, N., Tomos, P., Kakaris, S., Kosmas, C., Tsavaris, N."Distinct patterns of angiogenic factor expression as a predictive factor of response to chemotherapy in stage IIIA non‑small‑cell lung cancer patients". Molecular and Clinical Oncology 5, no. 4 (2016): 440-446. https://doi.org/10.3892/mco.2016.966
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