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Article

Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism

  • Authors:
    • Mohammad Hashemi
    • Gholamreza Bahari
    • Hedieh Sattarifard
    • Behzad Narouie
  • View Affiliations / Copyright

    Affiliations: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Urology and Nephrology Research Center, Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran 198396‑3113, Iran
  • Pages: 696-700
    |
    Published online on: August 8, 2017
       https://doi.org/10.3892/mco.2017.1369
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Abstract

The present study aimed to examine the impact of a 3-bp indel (rs57408770) polymorphism within the pre‑microRNA (miR)‑3131 polymorphism on prostate cancer (PCa) risk in a sample of an Iranian population. In total, 340 subjects, including 177 patients with PCa and 170 patients with benign prostatic hyperplasia, were enrolled in the present case‑control study. A mismatch polymerase chain reaction‑restriction fragment length polymorphism method was designed for genotyping the 3‑bp indel (rs57408770) polymorphism. The present findings demonstrated that the indel variant significantly increased the risk of PCa in codominant [odds ratio (OR)=2.23, 95% confidence interval (CI)=1.13‑4.37; P=0.021, insertion (ins)/ins vs. deletion (del)/del] and recessive (OR=2.33, 95% CI=1.25‑4.36; P=0.009, ins/ins vs. del/del + del/ins). In conclusion, to the best of our knowledge, the present findings for the first time proposed that a 3‑bp indel variant of miR‑3131 may be a risk factor for susceptibility to PCa in a sample of an Iranian population. Further studies with different ethnicities and larger sample sizes are required to validate the present findings.
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Copy and paste a formatted citation
Spandidos Publications style
Hashemi M, Bahari G, Sattarifard H and Narouie B: Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Mol Clin Oncol 7: 696-700, 2017.
APA
Hashemi, M., Bahari, G., Sattarifard, H., & Narouie, B. (2017). Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Molecular and Clinical Oncology, 7, 696-700. https://doi.org/10.3892/mco.2017.1369
MLA
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7.4 (2017): 696-700.
Chicago
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7, no. 4 (2017): 696-700. https://doi.org/10.3892/mco.2017.1369
Copy and paste a formatted citation
x
Spandidos Publications style
Hashemi M, Bahari G, Sattarifard H and Narouie B: Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Mol Clin Oncol 7: 696-700, 2017.
APA
Hashemi, M., Bahari, G., Sattarifard, H., & Narouie, B. (2017). Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Molecular and Clinical Oncology, 7, 696-700. https://doi.org/10.3892/mco.2017.1369
MLA
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7.4 (2017): 696-700.
Chicago
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7, no. 4 (2017): 696-700. https://doi.org/10.3892/mco.2017.1369
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