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Molecular and Clinical Oncology
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October-2018 Volume 9 Issue 4

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Article

High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma

  • Authors:
    • Jian Qin
    • Min Wang
    • Jun Qin
    • Qu Cai
    • Zhi‑Hai Peng
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, First People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200080, P.R. China, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 467-471
    |
    Published online on: August 16, 2018
       https://doi.org/10.3892/mco.2018.1700
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Abstract

Conditionally replicating adenoviral vectors constructed with tumor‑specific promoters (TSPs) offers a viable tool for the treatment of cholangiocarcinoma. The aim of the present study was to investigate cholangiocarcinoma‑specific TSPs that remain active in adenoviral constructs in gene therapy. The mRNA expressions of cyclooxygenase‑2, cytokeratin‑19 (CK19), mucin‑1, midkine and telomerase reverse transcriptase were determined in human cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using reverse transcription‑quantitative polymerase chain reaction. The candidate promoters constructed in adenoviral vectors were analyzed for their activities in cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using dual‑luciferase reporter assays. The mRNA expression of CK19 was markedly higher in the QBC939 cell line, indicating specificity to cholangiocarcinoma. Moreover, the promoter activity of CK19 in the adenoviral vector in infected cholangiocarcinoma cells was found to be significantly stronger compared with that in infected primary human hepatocytes and cholangiocytes. CK19 may be implicated in the pathogenesis of cholangiocarcinoma, as demonstrated by the stronger activity of its promoter, as well as the higher expression of mRNA in tumor cells. Therefore, the use of the promoter sequence of the CK19 gene may represent a potential tool in cholangiocarcinoma‑specific adenoviral gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Qin J, Wang M, Qin J, Cai Q and Peng ZH: High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma. Mol Clin Oncol 9: 467-471, 2018.
APA
Qin, J., Wang, M., Qin, J., Cai, Q., & Peng, Z. (2018). High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma. Molecular and Clinical Oncology, 9, 467-471. https://doi.org/10.3892/mco.2018.1700
MLA
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9.4 (2018): 467-471.
Chicago
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9, no. 4 (2018): 467-471. https://doi.org/10.3892/mco.2018.1700
Copy and paste a formatted citation
x
Spandidos Publications style
Qin J, Wang M, Qin J, Cai Q and Peng ZH: High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma. Mol Clin Oncol 9: 467-471, 2018.
APA
Qin, J., Wang, M., Qin, J., Cai, Q., & Peng, Z. (2018). High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma. Molecular and Clinical Oncology, 9, 467-471. https://doi.org/10.3892/mco.2018.1700
MLA
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9.4 (2018): 467-471.
Chicago
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9, no. 4 (2018): 467-471. https://doi.org/10.3892/mco.2018.1700
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