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Article

Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation

  • Authors:
    • Gonca Hanedan Uslu
    • Emine Canyilmaz
    • Lasif Serdar
    • Şafak Ersöz
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Health Sciences University Kanuni Training and Research Hospital, Trabzon 61250, Turkey, Department of Radiation Oncology, Faculty of Medicine, Karadeniz Technical University, Trabzon 61080, Turkey, Department of Medical Pathology, Faculty of Medicine, Karadeniz Technical University, Trabzon 61080, Turkey
  • Pages: 261-266
    |
    Published online on: December 11, 2018
       https://doi.org/10.3892/mco.2018.1790
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Abstract

The aim of the present study was to investigate the effectiveness of melatonin and genistein in preventing radiation therapy (RT)‑induced liver injury in mice. A total of 70 Swiss Albino male mice were divided into 7 equal groups (n=10/group) as follows: Melatonin (M group, G3), genistein (G group, G4), polyethylene glycol‑400 (P group, G5), RT only (RT group, G2) and sham irradiation (C group, G1). RT plus genistein (RT+G group, G7) and RT plus melatonin (RT+M group, G6) were the co‑treatment groups. Firstly, hepatic tissue damage was induced in mice via exposure to a single dose of 6‑Gy irradiation. RT was performed with a cobalt‑60 teletherapy machine (80 cm fixed source‑to‑surface distance, 2.5‑cm depth). Melatonin was processed (100 mg/kg, intraperitoneal) 30 min before and genistein was administered (200 mg/kg, SC) one day prior to the single dose of irradiation. Six months following irradiation, all mice were sacrificed. The degree of liver injury was measured using histological liver sections. Liver injury was significantly worse in the RT group than in the control group (C; RT vs. C; P<0.05); however, liver injury decreased following co‑treatment with melatonin or genistein vs. RT alone (RT+M and RT+G vs. RT; P<0.05). No difference was observed between the RT+M and RT+G groups (P>0.05). The present study revealed that melatonin and genistein administration prior to irradiation protects mice against liver injury, which may have therapeutic implications for RT‑induced injuries.
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Copy and paste a formatted citation
Spandidos Publications style
Hanedan Uslu G, Canyilmaz E, Serdar L and Ersöz Ş: Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation. Mol Clin Oncol 10: 261-266, 2019.
APA
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., & Ersöz, Ş. (2019). Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation. Molecular and Clinical Oncology, 10, 261-266. https://doi.org/10.3892/mco.2018.1790
MLA
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., Ersöz, Ş."Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation". Molecular and Clinical Oncology 10.2 (2019): 261-266.
Chicago
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., Ersöz, Ş."Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation". Molecular and Clinical Oncology 10, no. 2 (2019): 261-266. https://doi.org/10.3892/mco.2018.1790
Copy and paste a formatted citation
x
Spandidos Publications style
Hanedan Uslu G, Canyilmaz E, Serdar L and Ersöz Ş: Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation. Mol Clin Oncol 10: 261-266, 2019.
APA
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., & Ersöz, Ş. (2019). Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation. Molecular and Clinical Oncology, 10, 261-266. https://doi.org/10.3892/mco.2018.1790
MLA
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., Ersöz, Ş."Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation". Molecular and Clinical Oncology 10.2 (2019): 261-266.
Chicago
Hanedan Uslu, G., Canyilmaz, E., Serdar, L., Ersöz, Ş."Protective effects of genistein and melatonin on mouse liver injury induced by whole‑body ionising radiation". Molecular and Clinical Oncology 10, no. 2 (2019): 261-266. https://doi.org/10.3892/mco.2018.1790
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