Open Access

A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation

  • Authors:
    • Laura Giunti
    • Martina Da Ros
    • Veronica De Gregorio
    • Alberto Magi
    • Samuela Landini
    • Benedetta Mazzinghi
    • Anna Maria Buccoliero
    • Lorenzo Genitori
    • Sabrina Giglio
    • Iacopo Sardi
  • View Affiliations

  • Published online on: January 2, 2019     https://doi.org/10.3892/mco.2019.1795
  • Pages: 331-338
  • Copyright: © Giunti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) are a novel class of gene regulators that may be involved in tumor chemoresistance. Recently, specific miRNA expression profiles have been identified in adult glioblastoma (aGBM), but there are only limited data available on the role of miRNAs in pediatric GBM (pGBM). In the present study, the expression profile of miRNAs was examined in seven pGBMs and three human GBM cell lines (U87MG, A172 and T98G), compared with a non‑tumoral pool of pediatric cerebral cortex samples by microarray analysis. A set of differentially expressed miRNAs was identified, including miR‑490, miR‑876‑3p, miR‑876‑5p, miR‑448 and miR‑137 (downregulated), as well as miR‑501‑3p (upregulated). Through bioinformatics analysis, a series of target genes was predicted. In addition, similar gene expression patterns in pGBMs and cell lines was confirmed. Of note, drug resistant T98G cells had upregulated nuclear casein kinase and cyclin‑dependent kinase substrate 1 (NUCKS1) expression, a protein overexpressed in many tumors that serves an important role in cell proliferation and progression. On the basis of the present preliminary report, it could be intriguing to further investigate the relationship between each of the identified differentially expressed miRNAs and NUCKS1, in order to clarify their involvement in the multi‑drug resistance mechanism of pGBMs.
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March-2019
Volume 10 Issue 3

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Giunti L, Da Ros M, De Gregorio V, Magi A, Landini S, Mazzinghi B, Buccoliero AM, Genitori L, Giglio S, Sardi I, Sardi I, et al: A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation. Mol Clin Oncol 10: 331-338, 2019
APA
Giunti, L., Da Ros, M., De Gregorio, V., Magi, A., Landini, S., Mazzinghi, B. ... Sardi, I. (2019). A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation. Molecular and Clinical Oncology, 10, 331-338. https://doi.org/10.3892/mco.2019.1795
MLA
Giunti, L., Da Ros, M., De Gregorio, V., Magi, A., Landini, S., Mazzinghi, B., Buccoliero, A. M., Genitori, L., Giglio, S., Sardi, I."A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation". Molecular and Clinical Oncology 10.3 (2019): 331-338.
Chicago
Giunti, L., Da Ros, M., De Gregorio, V., Magi, A., Landini, S., Mazzinghi, B., Buccoliero, A. M., Genitori, L., Giglio, S., Sardi, I."A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation". Molecular and Clinical Oncology 10, no. 3 (2019): 331-338. https://doi.org/10.3892/mco.2019.1795