Inhibitory effects of Euphorbia tirucalli latex on murine B16/F10 melanoma cells and lung metastasis

Brunetti,Rafael   Lanciani; Da Paz,Diego   Pinha Alves; Mackowiak Da Fonseca,Ivone   Izabel; Nagamine,Márcia   Kazumi; Mori,Claudia   Madalena Cabrera; Del Grande,Murilo   Penteado; Queiroz‑Hazarbassanov,Nicolle; Salles‑Gomes,Cristina   Oliveira Massoco; Dagli,Maria   Lucia Zaidan; Hernandez‑Blazquez,Francisco   Javier

doi:10.3892/mco.2019.1916

Inhibitory effects of Euphorbia tirucalli latex on murine B16/F10 melanoma cells and lung metastasis

Authors:
- Rafael Lanciani Brunetti
- Diego Pinha Alves Da Paz
- Ivone Izabel Mackowiak Da Fonseca
- Márcia Kazumi Nagamine
- Claudia Madalena Cabrera Mori
- Murilo Penteado Del Grande
- Nicolle Queiroz‑Hazarbassanov
- Cristina Oliveira Massoco Salles‑Gomes
- Maria Lucia Zaidan Dagli
- Francisco Javier Hernandez‑Blazquez
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Affiliations: Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508‑900, Brazil, Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508‑900, Brazil
Published online on: September 4, 2019 https://doi.org/10.3892/mco.2019.1916
Pages: 511-516

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Abstract

Euphorbia tirucalli (E. tirucalli) is a tropical and subtropical plant that produces a latex which is used for several purposes. The components of E. tirucalli latex include triterpenes, diterpenes and steroids. The aim of the present study was to evaluate the effects of diluted E. tirucalli latex on murine B16/F10 melanoma cells and lung metastasis. For this purpose, an in vitro study was first performed, in which B16/F10 cells were treated with diluted (1/2 to 1/11,192) E. tirucalli latex. In a second study, B16/F10 melanoma cells were inoculated into the tail vein of mice to generate lung metastases; the mice then received 0.467 µg of latex diluted in 200 ml saline by gavage for 14 days. A significant decrease in B16/F10 cell viability was observed using the MTT assay at 24 and 48 h after treatment with E. tirucalli latex. In addition, a significant decrease in the volume fraction occupied by B16/F10 metastatic colonies in the lungs was observed in mice treated with E. tirucalli latex. These results confirm the antineoplastic effects of diluted E. tirucalli latex.

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