Verteporfin‑photodynamic therapy is effective on gastric cancer cells

Mae,Yukari; Kanda,Tsutomu; Sugihara,Takaaki; Takata,Tomoaki; Kinoshita,Hidehito; Sakaguchi,Takuki; Hasegawa,Takashi; Tarumoto,Ryohei; Edano,Mirai; Kurumi,Hiroki; Ikebuchi,Yuichiro; Kawaguchi,Koichiro; Isomoto,Hajime

doi:10.3892/mco.2020.2081

September-2020 Volume 13 Issue 3

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September-2020 Volume 13 Issue 3

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Article

Verteporfin‑photodynamic therapy is effective on gastric cancer cells

Authors:
- Yukari Mae
- Tsutomu Kanda
- Takaaki Sugihara
- Tomoaki Takata
- Hidehito Kinoshita
- Takuki Sakaguchi
- Takashi Hasegawa
- Ryohei Tarumoto
- Mirai Edano
- Hiroki Kurumi
- Yuichiro Ikebuchi
- Koichiro Kawaguchi
- Hajime Isomoto
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Affiliations: Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Tottori 683‑8504, Japan
Article Number: 10
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Published online on: June 25, 2020

https://doi.org/10.3892/mco.2020.2081
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Abstract

Photodynamic therapy (PDT) induces photochemical reactions, resulting in the destruction of tumor cells via singlet (S1) oxygen production. This cellular destruction occurs specifically in tumor cells, following selective accumulation of a photosensitizer and its excitation by a specific wavelength. Verteporfin (VP) is a second‑generation photosensitizer that is currently being used worldwide in PDT to treat age‑related macular degeneration. In addition, clinical trials with VP‑PDT demonstrated anti‑tumor efficacy and overall safety when used to treat locally advanced pancreatic cancer. In the present study, we examined the anti‑tumor effect of VP‑PDT on gastric cancer (GC) cell lines in vitro to conduct an initial assessment of its potential clinical applicability to this specific type of cancer. We evaluated the viability of MKN45 and MKN74 cancer cell lines after VP‑PDT exposure and calculated the half maximal effective concentration (EC₅₀) values for VP. Apoptosis in VP‑PDT‑exposed GC cells was observed. Furthermore, the EC₅₀ values for a 30‑min treatment with VP (2.5 J/cm² of 660 nm LED light) were 0.61 and 1.21 µM for MKN45 and MKN74, respectively. When VP treatment times were increased, the EC50 values decreased. In conclusion, VP‑PDT may be developed as an effective treatment for GC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Verteporfin‑photodynamic therapy is effective on gastric cancer cells

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