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Molecular and Clinical Oncology
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October-2021 Volume 15 Issue 4

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Article

A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results

  • Authors:
    • Carolina Ribeiro
    • Inês Gante
    • Margarida Figueiredo Dias
    • Ana Gomes
    • Henriqueta Coimbra Silva
  • View Affiliations / Copyright

    Affiliations: Institute of Medical Genetics/UC Genomics, Faculty of Medicine, University of Coimbra, 3000‑548 Coimbra, Portugal, University Clinic of Gynecology, Coimbra Hospital and University Centre (CHUC), 3000‑075 Coimbra, Portugal, Department of Pathology, Coimbra Hospital and University Centre (CHUC), 3000‑075 Coimbra, Portugal
  • Article Number: 216
    |
    Published online on: August 25, 2021
       https://doi.org/10.3892/mco.2021.2380
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Abstract

The one‑step nucleic acid amplification (OSNA) assay is a molecular method used for detecting breast cancer (BC) metastasis in sentinel lymph nodes (SLNs). However, this method has a major disadvantage, since it prevents tissue structure analysis, while only one molecular marker can be evaluated, namely cytokeratin 19 mRNA. The aim of the present study was to evaluate whether an OSNA‑discarded sample could be suitable for the gene expression analysis of the SLN microenvironment. The remaining intermediate phase of the centrifuged SLN homogenate obtained from the OSNA assay of samples from two patients with BC was used for mRNA extraction. Subsequently, the expression of five genes, namely forkhead box, cluster of differentiation 4 and three control genes, was determined by reverse transcription‑quantitative PCR analysis. The results demonstrated that high‑quality RNA was extracted. Therefore, this RNA may be used for gene expression analyses to predict novel molecular biomarkers associated with immuno‑inflammatory microenvironment.
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1 

Cardoso F, Kyriakides S, Ohno S, Penault-Llorca F, Poortmans P, Rubio IT, Zackrisson S and Senkus E: ESMO Guidelines Committee. Electronic address: simpleclinicalguidelines@esmo.org:. Early breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up+. Ann Oncol. 30:1194–1220. 2019.PubMed/NCBI View Article : Google Scholar

2 

Jimbo K, Kinoshita T, Suzuki J, Asaga S, Hojo T, Yoshida M and Tsuda H: Sentinel and nonsentinel lymph node assessment using a combination of one-step nucleic acid amplification and conventional histological examination. Breast. 22:1194–1199. 2013.PubMed/NCBI View Article : Google Scholar

3 

Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, Kato Y, Tsuda H, Ueda S, Sato K, et al: One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res. 13:4807–4816. 2007.PubMed/NCBI View Article : Google Scholar

4 

Banerjee SM, Michalopoulos NV, Williams NR, Davidson T, El Sheikh S, McDermott N, Tran-Dang MA, Davison S and Keshtgar MR: Detailed evaluation of one step nucleic acid (OSNA) molecular assay for intra-operative diagnosis of sentinel lymph node metastasis and prediction of non-sentinel nodal involvement: Experience from a London teaching hospital. Breast. 23:378–384. 2014.PubMed/NCBI View Article : Google Scholar

5 

Hunter-Smith AE and Rayter Z: One-step nucleic acid amplification: The possible value in assessing sentinel lymph node metastasis during mastectomy. Breast Cancer (Dove Med Press). 10:13–21. 2018.PubMed/NCBI View Article : Google Scholar

6 

Brito MJ, Honavar M, Cipriano MA, Lopes J, Coelho H, Silva AR, Silva M, Guimarães S, Frutuoso A, Gomes A, et al: Molecular staging of patients with colon cancer. The C-Closer-II Study: A multicentre study in Portugal. Acta Med Port. 31:661–669. 2018.PubMed/NCBI View Article : Google Scholar

7 

Gibert-Ramos A, López C, Bosch R, Fontoura L, Bueno G, García-Rojo M, Berenguer M and Lejeune M: Immune response profile of primary tumour, sentinel and non-sentinel axillary lymph nodes related to metastasis in breast cancer: An immunohistochemical point of view. Histochem Cell Biol. 152:177–193. 2019.PubMed/NCBI View Article : Google Scholar

8 

Grigoriadis A, Gazinska P, Pai T, Irhsad S, Wu Y, Millis R, Naidoo K, Owen J, Gillett CE, Tutt A, et al: Histological scoring of immune and stromal features in breast and axillary lymph nodes is prognostic for distant metastasis in lymph node-positive breast cancers. J Pathol Clin Res. 4:39–54. 2018.PubMed/NCBI View Article : Google Scholar

9 

Shiota T, Miyasato Y, Ohnishi K, Yamamoto-Ibusuki M, Yamamoto Y, Iwase H, Takeya M and Komohara Y: The Clinical Significance of CD169-Positive lymph node macrophage in patients with breast cancer. PLoS One. 11(e0166680)2016.PubMed/NCBI View Article : Google Scholar

10 

Martín-Sánchez E, Pernaut-Leza E, Mendaza S, Cordoba A, Vicente-Garcia F, Monreal-Santesteban I, Vizcaino JP, De Cerio MJ, Perez-Janices N, Blanco-Luquin I, et al: Gene promoter hypermethylation is found in sentinel lymph nodes of breast cancer patients, in samples identified as positive by one-step nucleic acid amplification of cytokeratin 19 mRNA. Virchows Arch. 469:51–59. 2016.PubMed/NCBI View Article : Google Scholar

11 

Gallego Romero I, Pai AA, Tung J and Gilad Y: RNA-seq: Impact of RNA degradation on transcript quantification. BMC Biol. 12(42)2014.PubMed/NCBI View Article : Google Scholar

12 

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, et al: The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 55:611–622. 2009.PubMed/NCBI View Article : Google Scholar

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

14 

Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, et al: Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics. 13:397–406. 2014.PubMed/NCBI View Article : Google Scholar

15 

Lu L, Barbi J and Pan F: The regulation of immune tolerance by FOXP3. Nat Rev Immunol. 17:703–717. 2017.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ribeiro C, Gante I, Dias MF, Gomes A and Silva HC: A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Mol Clin Oncol 15: 216, 2021.
APA
Ribeiro, C., Gante, I., Dias, M.F., Gomes, A., & Silva, H.C. (2021). A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Molecular and Clinical Oncology, 15, 216. https://doi.org/10.3892/mco.2021.2380
MLA
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15.4 (2021): 216.
Chicago
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15, no. 4 (2021): 216. https://doi.org/10.3892/mco.2021.2380
Copy and paste a formatted citation
x
Spandidos Publications style
Ribeiro C, Gante I, Dias MF, Gomes A and Silva HC: A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Mol Clin Oncol 15: 216, 2021.
APA
Ribeiro, C., Gante, I., Dias, M.F., Gomes, A., & Silva, H.C. (2021). A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Molecular and Clinical Oncology, 15, 216. https://doi.org/10.3892/mco.2021.2380
MLA
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15.4 (2021): 216.
Chicago
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15, no. 4 (2021): 216. https://doi.org/10.3892/mco.2021.2380
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