A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results

  • Authors:
    • Carolina Ribeiro
    • Inês Gante
    • Margarida Figueiredo Dias
    • Ana Gomes
    • Henriqueta Coimbra Silva
  • View Affiliations

  • Published online on: August 25, 2021     https://doi.org/10.3892/mco.2021.2380
  • Article Number: 216
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Abstract

The one‑step nucleic acid amplification (OSNA) assay is a molecular method used for detecting breast cancer (BC) metastasis in sentinel lymph nodes (SLNs). However, this method has a major disadvantage, since it prevents tissue structure analysis, while only one molecular marker can be evaluated, namely cytokeratin 19 mRNA. The aim of the present study was to evaluate whether an OSNA‑discarded sample could be suitable for the gene expression analysis of the SLN microenvironment. The remaining intermediate phase of the centrifuged SLN homogenate obtained from the OSNA assay of samples from two patients with BC was used for mRNA extraction. Subsequently, the expression of five genes, namely forkhead box, cluster of differentiation 4 and three control genes, was determined by reverse transcription‑quantitative PCR analysis. The results demonstrated that high‑quality RNA was extracted. Therefore, this RNA may be used for gene expression analyses to predict novel molecular biomarkers associated with immuno‑inflammatory microenvironment.
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October-2021
Volume 15 Issue 4

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Ribeiro C, Gante I, Dias MF, Gomes A and Silva HC: A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Mol Clin Oncol 15: 216, 2021
APA
Ribeiro, C., Gante, I., Dias, M.F., Gomes, A., & Silva, H.C. (2021). A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results. Molecular and Clinical Oncology, 15, 216. https://doi.org/10.3892/mco.2021.2380
MLA
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15.4 (2021): 216.
Chicago
Ribeiro, C., Gante, I., Dias, M. F., Gomes, A., Silva, H. C."A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results". Molecular and Clinical Oncology 15, no. 4 (2021): 216. https://doi.org/10.3892/mco.2021.2380