Molecular cytogenetic characterization of the urethane-induced murine lung cell line LA-4 as a model for human squamous cell lung cancer
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- Published online on: November 14, 2021 https://doi.org/10.3892/mco.2021.2440
- Article Number: 9
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Copyright: © Azawi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
The murine tumor cell line LA‑4 (also known as LA4 or LA 4) has been extensively used in ~70 studies from 1975 to present. However, the genetic characteristics have not been comprehensively delineated, apart from a single solid‑stain cytogenetic study, which reported an average of 116 chromosomes per cell. LA‑4 was created via urethane induction in an A/He mouse and demonstrated characteristic features of lung adenoma cells. In the present study, multicolor banding‑based molecular cytogenetics was combined with molecular karyotyping to characterize ploidy, copy number alterations and chromosomal breakpoints of LA‑4. A hyper‑tetraploid karyotype with 85‑93 chromosomes per cell, three distinct (pseudo‑) dicentric derivatives, two neocentrics, four unbalanced translocations, two chromosomes with terminal deletions and one chromosome with a balanced inversion were detected. The results were translated into the human genome and a comparison with the literature revealed that LA‑4 is well‑suited as a murine model for human squamous cell lung cancer.
