Molecular cytogenetic characterization of the urethane-induced murine lung cell line LA-4 as a model for human squamous cell lung cancer

Azawi,Shaymaa; Balachandran,Milash; Kramer,Fritz; Kankel,Stefanie; Rincic,Martina; Liehr,Thomas

doi:10.3892/mco.2021.2440

January-2022 Volume 16 Issue 1

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January-2022 Volume 16 Issue 1

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Molecular cytogenetic characterization of the urethane-induced murine lung cell line LA-4 as a model for human squamous cell lung cancer

Authors:
- Shaymaa Azawi
- Milash Balachandran
- Fritz Kramer
- Stefanie Kankel
- Martina Rincic
- Thomas Liehr
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Affiliations: Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, D-07747 Jena, Germany, Croatian Institute for Brain Research, School of Medicine University of Zagreb, 10000 Zagreb, Croatia

Copyright: © Azawi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 9
|
Published online on: November 14, 2021

https://doi.org/10.3892/mco.2021.2440
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Abstract

The murine tumor cell line LA‑4 (also known as LA4 or LA 4) has been extensively used in ~70 studies from 1975 to present. However, the genetic characteristics have not been comprehensively delineated, apart from a single solid‑stain cytogenetic study, which reported an average of 116 chromosomes per cell. LA‑4 was created via urethane induction in an A/He mouse and demonstrated characteristic features of lung adenoma cells. In the present study, multicolor banding‑based molecular cytogenetics was combined with molecular karyotyping to characterize ploidy, copy number alterations and chromosomal breakpoints of LA‑4. A hyper‑tetraploid karyotype with 85‑93 chromosomes per cell, three distinct (pseudo‑) dicentric derivatives, two neocentrics, four unbalanced translocations, two chromosomes with terminal deletions and one chromosome with a balanced inversion were detected. The results were translated into the human genome and a comparison with the literature revealed that LA‑4 is well‑suited as a murine model for human squamous cell lung cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Molecular cytogenetic characterization of the urethane-induced murine lung cell line LA-4 as a model for human squamous cell lung cancer

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