Effects of endoplasmic reticulum stress‑mediated CREB3L1 on apoptosis of glioma cells

  • Authors:
    • Zhao Yan
    • Yaxin Hu
    • Yiwei Zhang
    • Qian Pu
    • Liangzhao Chu
    • Jian Liu
  • View Affiliations

  • Published online on: February 15, 2022     https://doi.org/10.3892/mco.2022.2516
  • Article Number: 83
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Abstract

The association between endoplasmic reticulum stress (ERS) and apoptosis has been extensively studied. Cyclic adenosine monophosphate responsive element binding protein 3 like 1 (CREB3L1) has an important role in the development of glioma. In the present study, the potential association between ERS‑induced apoptosis and CREB3L1 and its clinical implications were investigated. From a total of 30 cases, brain gliomas with different pathological grades surgically resected at the Department of Neurosurgery of the Affiliated Hospital of Guizhou Medical University (Guiyang, China) between January 2018 and January 2019 were collected. The expression of CREB3L1 and ERS‑related proteins in gliomas with different degrees of malignancy was detected by immunohistochemistry. Furthermore, U87‑MG glioma cells were cultured in vitro and treated with different concentrations of ERS inducer thapsigargin (TG). The Cell Counting Kit‑8 (CCK‑8) assay was performed to detect changes in cell activity at different incubation times and drug concentrations. Cell apoptosis was detected by Annexin Ⅴ‑FITC/propidium iodide double staining and the protein expression levels of CREB3L1 and ERS were detected by western blot analysis. Immunohistochemical analysis suggested that the expression levels of CREB3L1, glucose‑regulated protein, 78 kDa (GRP78) and C/EBP‑homologous protein (CHOP) in World Health Organization (WHO) grade I glioma were higher than those in WHO grade Ⅱ‑Ⅳ (all P<0.01). The results of the CCK‑8 assay suggested that the activity of U87‑MG glioma cells was significantly decreased after treatment with TG (all P<0.05), and this effect was time‑ and drug concentration‑dependent. Flow cytometric analysis indicated that the apoptotic rate of the cells was increased, which was significant when the concentration of TG was 0.1 µmol/l (P<0.01). Furthermore, the protein expression of CREB3L1, GRP78 and CHOP in glioma cells treated with TG was increased (P<0.05). However, the expression level of Bcl‑2 decreased (P<0.05). In conclusion, ERS may reduce the cell proliferative activity and promote apoptosis through mediating CREB3L1 expression. CREB3L1 may be a novel potential target for glioma therapy.
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April-2022
Volume 16 Issue 4

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Spandidos Publications style
Yan Z, Hu Y, Zhang Y, Pu Q, Chu L and Liu J: Effects of endoplasmic reticulum stress‑mediated CREB3L1 on apoptosis of glioma cells. Mol Clin Oncol 16: 83, 2022
APA
Yan, Z., Hu, Y., Zhang, Y., Pu, Q., Chu, L., & Liu, J. (2022). Effects of endoplasmic reticulum stress‑mediated CREB3L1 on apoptosis of glioma cells. Molecular and Clinical Oncology, 16, 83. https://doi.org/10.3892/mco.2022.2516
MLA
Yan, Z., Hu, Y., Zhang, Y., Pu, Q., Chu, L., Liu, J."Effects of endoplasmic reticulum stress‑mediated CREB3L1 on apoptosis of glioma cells". Molecular and Clinical Oncology 16.4 (2022): 83.
Chicago
Yan, Z., Hu, Y., Zhang, Y., Pu, Q., Chu, L., Liu, J."Effects of endoplasmic reticulum stress‑mediated CREB3L1 on apoptosis of glioma cells". Molecular and Clinical Oncology 16, no. 4 (2022): 83. https://doi.org/10.3892/mco.2022.2516