Open Access

Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancer

  • Authors:
    • Bruna Cristina Borges
    • Pedro Augusto Do Amaral
    • Luiz Ricardo Soldi
    • Victor Luigi Costa Silva
    • Fernanda Carvalho De Souza
    • Felipe Andrés Cordeiro Da Luz
    • Rogério Agenor De Araújo
    • Marcelo José Barbosa Silva
  • View Affiliations

  • Published online on: February 25, 2022     https://doi.org/10.3892/mco.2022.2526
  • Article Number: 93
  • Copyright: © Borges et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Klotho, a cellular anti‑senescence protein, is related to antitumor actions, growth regulation, proliferation and invasiveness in several types of tumor, including breast cancer. The present study aimed to analyze the serum levels of αKlotho in patients with breast cancer according to histopathological and immunohistochemical variables. A total of 74 patients and 60 healthy controls were recruited. Peripheral blood samples were collected and serum levels were assessed by sandwich ELISA. Clinical and diagnostic data were obtained from medical records and databases of the Clinical Hospital of the Federal University of Uberlândia (Uberlândia, Brazil). The results indicated no difference in the levels of αKlotho between patients and controls (P=0.068); however, the number of patients with breast cancer with undetectable αKlotho was high (n=52). Thus, the variables that were associated with the lowest survival rates were analyzed, relating them to undetectable αKlotho. Among cases of metastatic tumors or tumors with poor differentiation, positive lymph node status and triple‑negative status, patients with undetectable αKlotho predominated and had unfavorable overall survival. Due to the significant results obtained in triple‑negative patients, an in vitro analysis was performed to determine whether estrogen receptors (ERs) have a role in αKlotho production. Treatment of MCF‑7 cells with ER agonists, estradiol (E2) and diarylpropionitrile (DPN), resulted in increases in αKlotho expression and supernatant levels of both agonists, demonstrating a direct association between the ER and Klotho production; of note, the ERβ‑specific agonist DPN tripled αKlotho expression when compared to E2 (P=0.078). These data suggested that undetectable αKlotho in the serum of patients with breast cancer is related to unfavorable histopathological variables and poor prognosis and ERs possibly have an important role in maintaining adequate quantities of αKlotho.
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April-2022
Volume 16 Issue 4

Print ISSN: 2049-9450
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Spandidos Publications style
Borges BC, Do Amaral PA, Soldi LR, Silva VL, De Souza FC, Cordeiro Da Luz FA, De Araújo RA and Silva MJ: Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancer. Mol Clin Oncol 16: 93, 2022
APA
Borges, B.C., Do Amaral, P.A., Soldi, L.R., Silva, V.L., De Souza, F.C., Cordeiro Da Luz, F.A. ... Silva, M.J. (2022). Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancer. Molecular and Clinical Oncology, 16, 93. https://doi.org/10.3892/mco.2022.2526
MLA
Borges, B. C., Do Amaral, P. A., Soldi, L. R., Silva, V. L., De Souza, F. C., Cordeiro Da Luz, F. A., De Araújo, R. A., Silva, M. J."Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancer". Molecular and Clinical Oncology 16.4 (2022): 93.
Chicago
Borges, B. C., Do Amaral, P. A., Soldi, L. R., Silva, V. L., De Souza, F. C., Cordeiro Da Luz, F. A., De Araújo, R. A., Silva, M. J."Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancer". Molecular and Clinical Oncology 16, no. 4 (2022): 93. https://doi.org/10.3892/mco.2022.2526