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miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma

  • Authors:
    • Shigeatsu Takamizawa
    • Junya Kojima
    • Tomohiro Umezu
    • Masahiko Kuroda
    • Shigehiro Hayashi
    • Takenori Maruta
    • Aikou Okamoto
    • Hirotaka Nishi
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo 160‑0023, Japan, Department of Molecular Pathology, Tokyo Medical University, Tokyo 160‑8402, Japan, Department of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo 105‑8461, Japan
    Copyright: © Takamizawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 14
    |
    Published online on: December 28, 2023
       https://doi.org/10.3892/mco.2023.2712
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Abstract

Ovarian cancer is a malignant gynecologic disease rarely diagnosed in the early stages. Among the various types of ovarian cancer, clear cell carcinoma has a poor prognosis due to its malignant potential. MicroRNAs (miRNAs/miRs) regulate gene expression in cells by suppressing the translation of target genes or by degrading the target mRNA. miRNAs are also secreted from the cells in the blood, binding to proteins or lipids and assisting in cell‑cell communication. Therefore, serum miRNAs may be considered potential diagnostic biomarkers for ovarian cancer. The present study investigated and identified specific miRNAs associated with ovarian clear cell carcinoma and compared them to those in ovarian endometrioma samples and healthy controls. CA125, an ovarian tumor marker, did not differ between patients with ovarian clear cell carcinoma, endometriosis or healthy controls. Subsequently, four miRNAs (miR‑146a‑5p, miR‑191‑5p, miR‑484 and miR‑574‑3p) were analyzed. The expression levels of miR‑146a‑5p and miR‑191‑5p were significantly increased in the serum samples from patients with ovarian clear cell carcinoma compared with those in the healthy controls, but there was no significant difference compared with in patients with endometriosis. Furthermore, the bioinformatics analysis showed that CCND2 and NOTCH2 were the candidate target genes of miR‑146a‑5p and miR‑191‑5p. In conclusion, the results of the present study demonstrated that miR‑146a‑5p and miR‑191‑5p may be useful as early and non‑invasive diagnostic tools in ovarian clear cell carcinoma. These miRNAs can help in distinguishing between ovarian clear cell carcinoma and ovarian endometrioma. To the best of our knowledge, no previous studies have screened any candidates specifically for ovarian clear cell carcinoma.
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1 

Webb PM and Jordan SJ: Epidemiology of epithelial ovarian cancer. Best Pract Res Clin Obstet Gynaecol. 41:3–14. 2017.PubMed/NCBI View Article : Google Scholar

2 

Cheung A, Shah S, Parker J, Soor P, Limbu A, Sheriff M and Boussios S: Non-epithelial ovarian cancers: How much do we really know? Int J Environ Res Public Health. 19(1106)2022.PubMed/NCBI View Article : Google Scholar

3 

Howlader N, Noone AM, Krapcho M, Miller D, Brest A, Yu M, Ruhl J, Tatalovich Z, Mariotto A, Lewis DR (eds), et al: SEER cancer statistics review, 1975-2017, National Cancer Institute. Bethesda, MD, 2020. https://seer.cancer.gov/csr/1975_2017.

4 

Landrum LM, Java J, Mathews CA, Lanneau GS Jr, Copeland LJ, Armstrong DK and Walker JL: Prognostic factors for stage III epithelial ovarian cancer treated with intraperitoneal chemotherapy: A gynecologic oncology group study. Gynecol Oncol. 130:12–18. 2013.PubMed/NCBI View Article : Google Scholar

5 

Boussios S, Rassy E, Moschetta M, Ghose A, Adeleke S, Sanchez E, Sheriff M, Chargari C and Pavlidis N: BRCA mutations in ovarian and prostate cancer: Bench to bedside. Cancers (Basel). 14(3888)2022.PubMed/NCBI View Article : Google Scholar

6 

Trimble EL, Christan MC and Korsay C: Surgical debulking plus paclitaxel-based adjuvant chemotherapy superior to previous ovarian cancer therapies. Oncology. 13(1068)1999.

7 

Pavlidis N, Rassy E, Vermorken JB, Assi T, Kattan J, Boussios S and Smith-Gagen J: The outcome of patients with serous papillary peritoneal cancer, fallopian tube cancer, and epithelial ovarian cancer by treatment eras: 27 Years data from the SEER registry. Cancer Epidemiol. 75(102045)2021.PubMed/NCBI View Article : Google Scholar

8 

del Carmen MG, Birrer M and Schorge JO: Clear cell carcinoma of the ovary: A review of the literature. Gynecol Oncol. 126:481–490. 2012.PubMed/NCBI View Article : Google Scholar

9 

Duffy MJ, Bonfrer JM, Kulpa J, Rustin GJ, Soletormos G, Torre GC, Tuxen MK and Zwirner M: CA125 in ovarian cancer: European group on tumor markers guidelines for clinical use. Int J Gynecol Cancer. 15:679–691. 2005.PubMed/NCBI View Article : Google Scholar

10 

Tian C, Markman M, Zaino R, Ozols RF, McGuire WP, Muggia FM, Rose PG, Spriggs D and Armstrong DK: CA-125 change after chemotherapy in prediction of treatment outcome among advanced mucinous and clear cell epithelial ovarian cancers: A gynecologic oncology group study. Cancer. 115:1395–1403. 2009.PubMed/NCBI View Article : Google Scholar

11 

Ghose A, Bolina A, Mahajan I, Raza SA, Clarke M, Pal A, Sanchez E, Rallis KS and Boussios S: Hereditary ovarian cancer: Towards a cost-effective prevention strategy. Int J Environ Res Public Health. 19(12057)2022.PubMed/NCBI View Article : Google Scholar

12 

Kim VN, Han J and Siomi MC: Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol. 10:126–139. 2009.PubMed/NCBI View Article : Google Scholar

13 

Kosaka N, Yoshioka Y, Fujita Y and Ochiya T: Versatile roles of extracellular vesicles in cancer. J Clin Invest. 126:1163–1172. 2016.PubMed/NCBI View Article : Google Scholar

14 

Kim VN: MicroRNA biogenesis: Coordinated cropping and dicing. Nat Rev Mol Cell Biol. 6:376–385. 2005.PubMed/NCBI View Article : Google Scholar

15 

Pritchard CC, Cheng HH and Tewari M: MicroRNA profiling: approaches and considerations. Nat Rev Genet. 13:358–369. 2012.PubMed/NCBI View Article : Google Scholar

16 

Cortez MA, Bueso-Ramos C, Ferdin J, Lopez-Berestein G, Sood AK and Calin GA: MicroRNAs in body fluids-the mix of hormones and biomarkers. Nat Rev Clin Oncol. 8:467–477. 2011.PubMed/NCBI View Article : Google Scholar

17 

Nagamitsu Y, Nishi H, Sasaki T, Takaesu Y, Terauchi F and Isaka K: Profiling analysis of circulating microRNA expression in cervical cancer. Mol Clin Oncol. 5:189–194. 2016.PubMed/NCBI View Article : Google Scholar

18 

Yokoi A, Matsuzaki J, Yamamoto Y, Yoneoka Y, Takahashi K, Shimizu H, Uehara T, Ishikawa M, Ikeda SI, Sonoda T, et al: Integrated extracellular microRNA profiling for ovarian cancer screening. Nat Commun. 9(4319)2018.PubMed/NCBI View Article : Google Scholar

19 

Gurung A, Hung T, Morin J and Gilks CB: Molecular abnormalities in ovarian carcinoma: Clinical, morphological and therapeutic correlates. Histopathology. 62:59–70. 2013.PubMed/NCBI View Article : Google Scholar

20 

Grandi G, Toss A, Cortesi L, Botticelli L, Volpe A and Cagnacci A: The association between endometriomas and ovarian cancer: Preventive effect of inhibiting ovulation and menstruation during reproductive life. Biomed Res Int. 2015(751571)2015.PubMed/NCBI View Article : Google Scholar

21 

Schrauder MG, Strick R, Schulz-Wendtland R, Strissel PL, Kahmann L, Loehberg CR, Lux MP, Jud SM, Hartmann A, Hein A, et al: Circulating micro-RNAs as potential blood-based markers for early stage breast cancer detection. PLoS One. 7(e29770)2012.PubMed/NCBI View Article : Google Scholar

22 

Ohyashiki K, Umezu T, Yoshizawa SI, Ito Y, Ohyashiki M, Kawashima H, Tanaka M, Kuroda M and Ohyashiki JH: Clinical impact of down-regulated plasma miR-92a levels in non-Hodgkin's lymphoma. PLoS One. 6(e16408)2011.PubMed/NCBI View Article : Google Scholar

23 

Huang HY, Lin YC, Li J, Huang KY, Shrestha S, Hong HC, Tang Y, Chen YG, Jin CN, Yu Y, et al: miRTarBase 2020: Updates to the experimentally validated microRNA-target interaction database. Nucleic Acids Res. 48 (D1):D148–D154. 2020.PubMed/NCBI View Article : Google Scholar

24 

Shah S, Cheung A, Kutka M, Sheriff M and Boussios S: Epithelial ovarian cancer: Providing evidence of predisposition genes. Int J Environ Res Public Health. 19(8113)2022.PubMed/NCBI View Article : Google Scholar

25 

Ghose A, Gullapalli SVN, Chohan N, Bolina A, Moschetta M, Rassy E and Boussios S: Applications of proteomics in ovarian cancer: Dawn of a new era. Proteomes. 10(16)2022.PubMed/NCBI View Article : Google Scholar

26 

Taniguchi F: New knowledge and insights about the malignant transformation of endometriosis. J Obstet Gynaecol Res. 43:1093–1100. 2017.PubMed/NCBI View Article : Google Scholar

27 

Hua M, Qin Y, Sheng M, Cui X, Chen W, Zhong J, Yan J and Chen Y: miR-145 suppresses ovarian cancer progression via modulation of cell growth and invasion by targeting CCND2 and E2F3. Mol Med Rep. 19:3575–3583. 2019.PubMed/NCBI View Article : Google Scholar

28 

Kato JY and Sherr CJ: Inhibition of granulocyte differentiation by G1 cyclins D2 and D3 but not D1. Proc Natl Acad Sci USA. 90:11513–11517. 1993.PubMed/NCBI View Article : Google Scholar

29 

Song H, Hogdall E, Ramus SJ, Dicioccio RA, Hogdall C, Quaye L, McGuire V, Whittemore AS, Shah M, Greenberg D, et al: Effects of common germ-line genetic variation in cell cycle genes on ovarian cancer survival. Clin Cancer Res. 14:1090–1095. 2008.PubMed/NCBI View Article : Google Scholar

30 

Zhu H, Dougherty U, Robinson V, Mustafi R, Pekow J, Kupfer S, Li YC, Hart J, Goss K, Fichera A, et al: EGFR signals downregulate tumor suppressors miR-143 and miR-145 in Western diet-promoted murine colon cancer: Role of G1 regulators. Mol Cancer Res. 9:960–975. 2011.PubMed/NCBI View Article : Google Scholar

31 

Chang L, Guo R, Yuan Z, Shi H and Zhang D: LncRNA HOTAIR regulates CCND1 and CCND2 expression by sponging miR-206 in ovarian cancer. Cell Physiol Biochem. 49:1289–1303. 2018.PubMed/NCBI View Article : Google Scholar

32 

Lu S, Liu W, Shi H and Zhou H: Exosomal miR-34b inhibits proliferation and the epithelial-mesenchymal transition by targeting Notch2 in ovarian cancer. Oncol Lett. 20:2721–2728. 2020.PubMed/NCBI View Article : Google Scholar

33 

Revythis A, Limbu A, Mikropoulos C, Ghose A, Sanchez E, Sheriff M and Boussios S: Recent insights into PARP and immuno-checkpoint inhibitors in epithelial ovarian cancer. Int J Environ Res Public Health. 19(8577)2022.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Takamizawa S, Kojima J, Umezu T, Kuroda M, Hayashi S, Maruta T, Okamoto A and Nishi H: miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma. Mol Clin Oncol 20: 14, 2024.
APA
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T. ... Nishi, H. (2024). miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma. Molecular and Clinical Oncology, 20, 14. https://doi.org/10.3892/mco.2023.2712
MLA
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T., Okamoto, A., Nishi, H."miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma". Molecular and Clinical Oncology 20.2 (2024): 14.
Chicago
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T., Okamoto, A., Nishi, H."miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma". Molecular and Clinical Oncology 20, no. 2 (2024): 14. https://doi.org/10.3892/mco.2023.2712
Copy and paste a formatted citation
x
Spandidos Publications style
Takamizawa S, Kojima J, Umezu T, Kuroda M, Hayashi S, Maruta T, Okamoto A and Nishi H: miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma. Mol Clin Oncol 20: 14, 2024.
APA
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T. ... Nishi, H. (2024). miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma. Molecular and Clinical Oncology, 20, 14. https://doi.org/10.3892/mco.2023.2712
MLA
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T., Okamoto, A., Nishi, H."miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma". Molecular and Clinical Oncology 20.2 (2024): 14.
Chicago
Takamizawa, S., Kojima, J., Umezu, T., Kuroda, M., Hayashi, S., Maruta, T., Okamoto, A., Nishi, H."miR‑146a‑5p and miR‑191‑5p as novel diagnostic marker candidates for ovarian clear cell carcinoma". Molecular and Clinical Oncology 20, no. 2 (2024): 14. https://doi.org/10.3892/mco.2023.2712
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