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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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July-August 2008 Volume 1 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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July-August 2008 Volume 1 Issue 4

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Article

Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells

  • Authors:
    • Qinjun Wei
    • Yajie Lu
    • Mei Pan
    • Yongquian Shu
    • Xin Cao

  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Nanjing Medical University, Nanjing 210029, P.R. China
  • Pages: 479-483
    |
    Published online on: July 1, 2008
       https://doi.org/10.3892/mmr.1.4.479
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Abstract

Up-regulation of the human prolactin receptor (hPRLR) has been implicated in aberrant signaling that may induce abnormal proliferation of breast epithelium. In this study, we evaluated the inhibition of cell proliferation by small-interfering RNA (siRNA) targeting of hPRLR, as well as the anti-tumor efficacy of this treatment on the T-47D breast cancer cell line. The hPRLR-targeted siRNA were chemically synthesized and constructed in a specific siRNA expression vector (pSilencer 2.0), the expression of hPRLR was analyzed by real-time quantitative PCR and Western blot analysis, growth inhibition was measured by MTT assay, and cell cycle analysis was carried out to determine the effect of siRNA treatment on T-47D cells. Our results indicate that the hPRLR siRNA plasmid markedly reduced PRLR gene expression, decreased the growth rate and reduced the frequency of T-47D cells in the G2/M phase, while significantly increasing cells in G0/G1. In summary, RNAi silencing of hPRLR gene expression specifically inhibited the proliferation of T-47D breast cancer cells. This implies that RNAi have therapeutic potential as a treatment for breast cancer by targeting the overexpression of hPRLR, suggesting that this gene product might be a potential therapeutic target.

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Copy and paste a formatted citation
Spandidos Publications style
Wei Q, Lu Y, Pan M, Shu Y and Cao X: Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells. Mol Med Rep 1: 479-483, 2008.
APA
Wei, Q., Lu, Y., Pan, M., Shu, Y., & Cao, X. (2008). Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells. Molecular Medicine Reports, 1, 479-483. https://doi.org/10.3892/mmr.1.4.479
MLA
Wei, Q., Lu, Y., Pan, M., Shu, Y., Cao, X."Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells". Molecular Medicine Reports 1.4 (2008): 479-483.
Chicago
Wei, Q., Lu, Y., Pan, M., Shu, Y., Cao, X."Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells". Molecular Medicine Reports 1, no. 4 (2008): 479-483. https://doi.org/10.3892/mmr.1.4.479
Copy and paste a formatted citation
x
Spandidos Publications style
Wei Q, Lu Y, Pan M, Shu Y and Cao X: Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells. Mol Med Rep 1: 479-483, 2008.
APA
Wei, Q., Lu, Y., Pan, M., Shu, Y., & Cao, X. (2008). Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells. Molecular Medicine Reports, 1, 479-483. https://doi.org/10.3892/mmr.1.4.479
MLA
Wei, Q., Lu, Y., Pan, M., Shu, Y., Cao, X."Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells". Molecular Medicine Reports 1.4 (2008): 479-483.
Chicago
Wei, Q., Lu, Y., Pan, M., Shu, Y., Cao, X."Gene silencing of hPRLR mRNA by RNA interference in human breast cancer cells". Molecular Medicine Reports 1, no. 4 (2008): 479-483. https://doi.org/10.3892/mmr.1.4.479
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