Tapping an unexploited repository: Carnoy's fixed cell pellets for proteomic biomarker research in leukemia
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- Published online on: July 1, 2008 https://doi.org/10.3892/mmr.1.4.585
- Pages: 585-587
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Abstract
For chromosomal analysis in tumor genetics, cells from blood and bone marrow are prepared and preserved virtually indefinitely in Carnoy's fixative (methanol/acetic acid). Numerous samples are stored unvalued in hospitals and institutes worldwide. We developed a method to analyze proteins from even a small amount of these cells by mass spectrometry using affinity chromatographic surfaces (SELDI), and demonstrated the application of proteomic biomarker research in cases of acute myeloid leukemia.