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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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September-October 2010 Volume 3 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September-October 2010 Volume 3 Issue 5

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Article

Methylation status of the PTEN gene in adenoid cystic carcinoma cells

  • Authors:
    • Xiaoping Fan
    • Bin Chen
    • Junli Xu
    • Huachang Zhang
    • Feng Deng
    • Xuerong Xiang

  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Periodontics and Oral Medicine, Stomatological Hospital Affiliated to Chongqing University of Medical Science, Chongqing 400015, P.R. China, Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 775-779
    |
    Published online on: July 26, 2010
       https://doi.org/10.3892/mmr.2010.337
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Abstract

The tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is deficient in various types of human tumors due to mutations or epigenetic alterations. PTEN promoter hypermethylation is a major epigenetic silencing mechanism leading to self-repression in these tumors. The present study aimed to investigate whether PTEN promoter methylation is involved in the regulation of the PTEN gene in adenoid cystic carcinoma (ACC) cells. The expression of PTEN in ACC-2 cells was found to be significantly lower than that in normal salivary gland epithelial cells using RT-PCR analysis. The existence of CpG island methylation in the PETN promoter region in ACC-2 cells was demonstrated by methylation-specific PCR (MSP) analysis and direct sequencing of MSP product. RT-PCR, Western blot analysis and luciferase assay showed that mRNA and protein expression and the promoter activity of PTEN in ACC-2 cells treated with the DNA methylation inhibitor 5-aza-2-deoxycytidine were significantly up-regulated in a time-dependent manner. These results indicate that the hypermethylation of the PTEN promoter region leads to lower expression of PTEN gene in ACC cells, which aids in the development of PTEN as a molecular marker for the early diagnosis of this carcinoma.

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Copy and paste a formatted citation
Spandidos Publications style
Fan X, Chen B, Xu J, Zhang H, Deng F and Xiang X: Methylation status of the PTEN gene in adenoid cystic carcinoma cells. Mol Med Rep 3: 775-779, 2010.
APA
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., & Xiang, X. (2010). Methylation status of the PTEN gene in adenoid cystic carcinoma cells. Molecular Medicine Reports, 3, 775-779. https://doi.org/10.3892/mmr.2010.337
MLA
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., Xiang, X."Methylation status of the PTEN gene in adenoid cystic carcinoma cells". Molecular Medicine Reports 3.5 (2010): 775-779.
Chicago
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., Xiang, X."Methylation status of the PTEN gene in adenoid cystic carcinoma cells". Molecular Medicine Reports 3, no. 5 (2010): 775-779. https://doi.org/10.3892/mmr.2010.337
Copy and paste a formatted citation
x
Spandidos Publications style
Fan X, Chen B, Xu J, Zhang H, Deng F and Xiang X: Methylation status of the PTEN gene in adenoid cystic carcinoma cells. Mol Med Rep 3: 775-779, 2010.
APA
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., & Xiang, X. (2010). Methylation status of the PTEN gene in adenoid cystic carcinoma cells. Molecular Medicine Reports, 3, 775-779. https://doi.org/10.3892/mmr.2010.337
MLA
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., Xiang, X."Methylation status of the PTEN gene in adenoid cystic carcinoma cells". Molecular Medicine Reports 3.5 (2010): 775-779.
Chicago
Fan, X., Chen, B., Xu, J., Zhang, H., Deng, F., Xiang, X."Methylation status of the PTEN gene in adenoid cystic carcinoma cells". Molecular Medicine Reports 3, no. 5 (2010): 775-779. https://doi.org/10.3892/mmr.2010.337
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