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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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May-June 2011 Volume 4 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29

  • Authors:
    • Hirotsugu Imaeda
    • Akira Andoh
    • Tomoki Aomatsu
    • Rie Osaki
    • Shigeki  Bamba
    • Osamu Inatomi
    • Tomoharu Shimizu
    • Yoshihide Fujiyama

  • View Affiliations / Copyright

    Affiliations: Department of Medicine, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan, Division of Mucosal Immunology, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan, Department of Surgery, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
  • Pages: 483-487
    |
    Published online on: February 18, 2011
       https://doi.org/10.3892/mmr.2011.442
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Abstract

Interleukin (IL)-32 plays a role in the pathophysiology of inflammatory bowel disease (IBD). We isolated a new isoform of the IL-32 transcript in the process of cloning the full-length IL-32 gene from human colonic subepithelial myofibroblasts (SEMFs). The expression of mRNA in the samples was assessed by RT-PCR and real-time PCR analyses. The PCR products from the IL-32 genes were ligated into the expression vector pIRESneo2. The new isoform of the IL-32 transcript (336 nucleotides) completely lacked exon 4 of the IL-32γ gene, and was 60 bp shorter than IL-32α. TNF-α induced the mRNA expression of the new IL-32 isoform in a dose- and time-dependent manner. Stable transfection of this new isoform significantly decreased TNF-α-induced IL-8 mRNA expression in HT-29 cells, but the expression of the IL-32α gene had no effect. The mRNA expression of this new isoform was significantly elevated in the inflamed mucosa of IBD patients. A new isoform of the IL-32 transcript may play an anti-inflammatory role in the inflamed mucosa of IBD.

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Copy and paste a formatted citation
Spandidos Publications style
Imaeda H, Andoh A, Aomatsu T, Osaki R, Bamba S, Inatomi O, Shimizu T and Fujiyama Y: A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29. Mol Med Rep 4: 483-487, 2011.
APA
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O. ... Fujiyama, Y. (2011). A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29. Molecular Medicine Reports, 4, 483-487. https://doi.org/10.3892/mmr.2011.442
MLA
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O., Shimizu, T., Fujiyama, Y."A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29". Molecular Medicine Reports 4.3 (2011): 483-487.
Chicago
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O., Shimizu, T., Fujiyama, Y."A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29". Molecular Medicine Reports 4, no. 3 (2011): 483-487. https://doi.org/10.3892/mmr.2011.442
Copy and paste a formatted citation
x
Spandidos Publications style
Imaeda H, Andoh A, Aomatsu T, Osaki R, Bamba S, Inatomi O, Shimizu T and Fujiyama Y: A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29. Mol Med Rep 4: 483-487, 2011.
APA
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O. ... Fujiyama, Y. (2011). A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29. Molecular Medicine Reports, 4, 483-487. https://doi.org/10.3892/mmr.2011.442
MLA
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O., Shimizu, T., Fujiyama, Y."A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29". Molecular Medicine Reports 4.3 (2011): 483-487.
Chicago
Imaeda, H., Andoh, A., Aomatsu, T., Osaki, R., Bamba, S., Inatomi, O., Shimizu, T., Fujiyama, Y."A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29". Molecular Medicine Reports 4, no. 3 (2011): 483-487. https://doi.org/10.3892/mmr.2011.442
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