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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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May-June 2011 Volume 4 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer

  • Authors:
    • Zhenhua Liu
    • Li Shen
    • Lan Xu
    • Xiaoya Sun
    • Jinwei Zhou
    • Shiliang Wu

  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Inspection, Suzhou Health College, Suzhou, Jiangsu 215001, P.R. China
  • Pages: 497-503
    |
    Published online on: March 4, 2011
       https://doi.org/10.3892/mmr.2011.448
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Abstract

β-1,3-N-acetylglucosaminyltransferase-8 (β3Gn-T8) is the most recently identified enzyme in the β3Gn-T family, but its biological function is poorly understood. To elucidate the effects of β3Gn-T8 on gastric cancer behavior, β3Gn-T8 was down-regulated in AGS cells using small interfering RNA (siRNA). The mRNA and protein expression levels of β3Gn-T8 were detected using RT-PCR and Western blotting, respectively, and sequence-specific inhibition using siRNA was also measured using RT-PCR in human SPCA-1 and SGC-7901 cells. The cell proliferation rate was determined using MTT and the percentage of apoptotic cells was measured using flow cytometry. AGS cells transfected with β3Gn-T8 siRNA were subcutaneously transplanted into nude mice and tumorigenicity was assessed. The siRNA efficiently suppressed β3Gn-T8 expression in AGS cells, and the down-regulation of β3Gn-T8 caused significant inhibition of tumor cell growth in vitro. The apoptotic rate of AGS cells increased to 10.13% 48 h after siRNA transfection, which was five times that of the control cells. Furthermore, the knockdown of β3Gn-T8 expression reduced the tumorigenicity of gastric cancer cells in nude mice, suggesting that β3Gn-T8 has potential as a gastric cancer therapeutic target.

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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Shen L, Xu L, Sun X, Zhou J and Wu S: Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer. Mol Med Rep 4: 497-503, 2011.
APA
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., & Wu, S. (2011). Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer. Molecular Medicine Reports, 4, 497-503. https://doi.org/10.3892/mmr.2011.448
MLA
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., Wu, S."Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer". Molecular Medicine Reports 4.3 (2011): 497-503.
Chicago
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., Wu, S."Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer". Molecular Medicine Reports 4, no. 3 (2011): 497-503. https://doi.org/10.3892/mmr.2011.448
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Shen L, Xu L, Sun X, Zhou J and Wu S: Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer. Mol Med Rep 4: 497-503, 2011.
APA
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., & Wu, S. (2011). Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer. Molecular Medicine Reports, 4, 497-503. https://doi.org/10.3892/mmr.2011.448
MLA
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., Wu, S."Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer". Molecular Medicine Reports 4.3 (2011): 497-503.
Chicago
Liu, Z., Shen, L., Xu, L., Sun, X., Zhou, J., Wu, S."Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer". Molecular Medicine Reports 4, no. 3 (2011): 497-503. https://doi.org/10.3892/mmr.2011.448
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