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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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May-June 2011 Volume 4 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

  • Authors:
    • Jian-Jiang Zhao
    • Jun Chen
    • Zhi-Ping Wang
    • Jie Pan
    • Yu-Hua Huang

  • View Affiliations / Copyright

    Affiliations: Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou 510280, P.R. China
  • Pages: 425-429
    |
    Published online on: March 15, 2011
       https://doi.org/10.3892/mmr.2011.457
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Abstract

The aim of this study was to evaluate the application of quantum dots (QDs) and the FITC labeling technique in Tca8113 cells, and to compare the fluorescence intensity and photostability of these techniques. First, using one-colour confocal microscopy, QDs and FITC were applied separately to tag HSP70 in Tca8113 strains with indirect immunofluorescence. The expression of HSP70 in Tca8113 cells was observed using confocal laser microscopy, and the fluorescence intensity between QDs and FITC was compared. Using two-colour confocal microscopy, QDs and FITC were applied to tag survivin and HSP70, respectively, in order to compare the fluorescence photostability between QDs and FITC. Using a laser scanning microscope, survivin and HSP70 expression was clearly detected in the Tca8113 cells. The strength of the fluorescence signal from QDs was higher than that from FITC. After 40 min of continuous laser exposure, there was no observable decline in the QD fluorescent markers, whereas the FITC fluorescent signals faded very quickly and became undetectable. QDs have better fluorescence intensity and photostability than FITC, and are more appropriate for studies that require a long dynamic observation of physiological changes in cells.

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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Chen J, Wang Z, Pan J and Huang Y: Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors. Mol Med Rep 4: 425-429, 2011.
APA
Zhao, J., Chen, J., Wang, Z., Pan, J., & Huang, Y. (2011). Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors. Molecular Medicine Reports, 4, 425-429. https://doi.org/10.3892/mmr.2011.457
MLA
Zhao, J., Chen, J., Wang, Z., Pan, J., Huang, Y."Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors". Molecular Medicine Reports 4.3 (2011): 425-429.
Chicago
Zhao, J., Chen, J., Wang, Z., Pan, J., Huang, Y."Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors". Molecular Medicine Reports 4, no. 3 (2011): 425-429. https://doi.org/10.3892/mmr.2011.457
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Chen J, Wang Z, Pan J and Huang Y: Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors. Mol Med Rep 4: 425-429, 2011.
APA
Zhao, J., Chen, J., Wang, Z., Pan, J., & Huang, Y. (2011). Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors. Molecular Medicine Reports, 4, 425-429. https://doi.org/10.3892/mmr.2011.457
MLA
Zhao, J., Chen, J., Wang, Z., Pan, J., Huang, Y."Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors". Molecular Medicine Reports 4.3 (2011): 425-429.
Chicago
Zhao, J., Chen, J., Wang, Z., Pan, J., Huang, Y."Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors". Molecular Medicine Reports 4, no. 3 (2011): 425-429. https://doi.org/10.3892/mmr.2011.457
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