RNA interference targeting NRP-1 inhibits human glioma cell proliferation and enhances cell apoptosis

  • Authors:
    • Xiangyun Li
    • Ting Tang
    • Xiaozhe Lu
    • Houguang Zhou
    • Yanyan Huang
  • View Affiliations

  • Published online on: August 12, 2011     https://doi.org/10.3892/mmr.2011.550
  • Pages:1261-1266
0

Abstract

Neuropilin-1 (NRP-1) is a non-tyrosine kinase receptor for vascular endothelial growth factor (VEGF) that was recently found to play a role in tumor functions. Previous studies demonstrated that NRP-1 was overexpressed in a number of human tumors, including glioblastoma (GBM). However, the role of NRP-1 in glioma progression has yet to be adequately elucidated. Thus, we examined the expression of NRP-1 in human glioma cell lines using Western blotting, and cell cycle distribution and proliferation by transfection of the U373 cell line with NRP-1 short interference RNA (siRNA). Results showed NRP-1 siRNA to significantly reduce NRP-1 gene expression, decrease in vitro cell proliferation and induce cell apoptosis in cultured glioma cells, along with the accumulation of cells in the G1 phase and a decrease in cells in the S phase. Our results further revealed that NRP-1 knockdown decreased the expression levels of Bcl-2 family proteins and deactivated extracellular signal-regulated kinase (ERK) and c-Jun‑N‑terminal kinase (JNK)/mitogen-activated protein kinase (MAPK) signaling pathways, closely associated with cancer progression. Thus, our results provide a molecular mechanism for the effect of NRP-1 in tumors, rendering NRP-1 an attractive candidate as a therapeutic target in certain types of cancer, such as GBM.

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November 2011
Volume 4 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Li, X., Tang, T., Lu, X., Zhou, H., & Huang, Y. (2011). RNA interference targeting NRP-1 inhibits human glioma cell proliferation and enhances cell apoptosis. Molecular Medicine Reports, 4, 1261-1266. https://doi.org/10.3892/mmr.2011.550
MLA
Li, X., Tang, T., Lu, X., Zhou, H., Huang, Y."RNA interference targeting NRP-1 inhibits human glioma cell proliferation and enhances cell apoptosis". Molecular Medicine Reports 4.6 (2011): 1261-1266.
Chicago
Li, X., Tang, T., Lu, X., Zhou, H., Huang, Y."RNA interference targeting NRP-1 inhibits human glioma cell proliferation and enhances cell apoptosis". Molecular Medicine Reports 4, no. 6 (2011): 1261-1266. https://doi.org/10.3892/mmr.2011.550