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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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March 2012 Volume 5 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation

  • Authors:
    • Dong-Li Zhao
    • Cheng-Xue Dang
    • Yan-Xia Sui
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, P.R. China
  • Pages: 866-872
    |
    Published online on: December 20, 2011
       https://doi.org/10.3892/mmr.2011.723
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Abstract

This study aimed to investigate the involvement of c-erbB-2, encoded by the receptor tyrosine kinase ERBB2 gene, in the pathogenesis of colorectal cancer and to validate its potential as an anticancer target. Immunohistochemical and histopathological analyses were applied in tissue samples derived from 80 colorectal cancer patients. ERBB2 stable small hairpin RNA (shRNA) knockdown in HT29 human colorectal cancer cells was confirmed by RT-PCR and western blotting. Cell cycle profile and apoptosis were measured using PI or Annexin V-PI dual staining. A significant correlation between ERBB2 levels and Dukes' stage of colorectal cancer, in both the primary malignancy and lymph node metastatic tissues, was observed. ERBB2-depleted HT-29 cells exhibited increased sensitivity to radiation compared to control cells, likely due to enhanced G0/G1 phase cell cycle arrest and apoptosis. ERBB2 may be involved in the malignancy and metastasis of colorectal cancer. Overexpressed ERBB2 may constitute a potential target for colorectal cancer therapy.

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Copy and paste a formatted citation
Spandidos Publications style
Zhao D, Dang C and Sui Y: RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation. Mol Med Rep 5: 866-872, 2012.
APA
Zhao, D., Dang, C., & Sui, Y. (2012). RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation. Molecular Medicine Reports, 5, 866-872. https://doi.org/10.3892/mmr.2011.723
MLA
Zhao, D., Dang, C., Sui, Y."RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation". Molecular Medicine Reports 5.3 (2012): 866-872.
Chicago
Zhao, D., Dang, C., Sui, Y."RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation". Molecular Medicine Reports 5, no. 3 (2012): 866-872. https://doi.org/10.3892/mmr.2011.723
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao D, Dang C and Sui Y: RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation. Mol Med Rep 5: 866-872, 2012.
APA
Zhao, D., Dang, C., & Sui, Y. (2012). RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation. Molecular Medicine Reports, 5, 866-872. https://doi.org/10.3892/mmr.2011.723
MLA
Zhao, D., Dang, C., Sui, Y."RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation". Molecular Medicine Reports 5.3 (2012): 866-872.
Chicago
Zhao, D., Dang, C., Sui, Y."RNAi-mediated ERBB2 gene knockdown sensitizes human colorectal cancer cells to radiation". Molecular Medicine Reports 5, no. 3 (2012): 866-872. https://doi.org/10.3892/mmr.2011.723
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