Sulforaphane protects against 6-hydroxydopamine-induced cytotoxicity by increasing expression of heme oxygenase-1 in a PI3K/Akt-dependent manner

  • Authors:
    • Chang Deng
    • Ran Tao
    • Shun-Zhi Yu
    • Hua Jin
  • View Affiliations

  • Published online on: December 21, 2011     https://doi.org/10.3892/mmr.2011.731
  • Pages: 847-851
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Abstract

Parkinson's disease (PD) is a progressive neurodegenerative disorder with selective loss of dopaminergic neurons in the substantia nigra. Evidence suggests that oxidative stress is involved in the pathogenesis of PD. Sulforaphane (SF), a naturally occurring isothiocyanate, has been shown to protect against oxidative stress by inducing the expression of various NF-E2-related factor-2 (Nrf2) responsive genes. Previous studies have shown that SF protects dopaminergic neurons against PD-related neurotoxin 6-hydroxydopamine (6-OHDA)-induced cytotoxicity. However, the molecular mechanisms by which SF protects against 6-OHDA-induced cytotoxicity are poorly elucidated. In this study, we found that pretreatment with SF significantly reduced 6-OHDA-induced caspase-3 activation and subsequent cell death. SF also increased heme oxygenase-1 (HO-1) expression, which conferred protection against 6-OHDA-induced cytotoxicity. Furthermore, SF induced the translocation of Nrf2 into the nucleus and activated PI3K/Akt, a pathway that is involved in SF-induced Nrf2 nuclear translocation, HO-1 expression and cytoprotection. These results suggest that SF inhibits 6-OHDA-induced cytotoxicity through increasing HO-1 expression in a PI3K/Akt-dependent manner.

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March 2012
Volume 5 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Deng C, Tao R, Yu S and Jin H: Sulforaphane protects against 6-hydroxydopamine-induced cytotoxicity by increasing expression of heme oxygenase-1 in a PI3K/Akt-dependent manner. Mol Med Rep 5: 847-851, 2012.
APA
Deng, C., Tao, R., Yu, S., & Jin, H. (2012). Sulforaphane protects against 6-hydroxydopamine-induced cytotoxicity by increasing expression of heme oxygenase-1 in a PI3K/Akt-dependent manner. Molecular Medicine Reports, 5, 847-851. https://doi.org/10.3892/mmr.2011.731
MLA
Deng, C., Tao, R., Yu, S., Jin, H."Sulforaphane protects against 6-hydroxydopamine-induced cytotoxicity by increasing expression of heme oxygenase-1 in a PI3K/Akt-dependent manner". Molecular Medicine Reports 5.3 (2012): 847-851.
Chicago
Deng, C., Tao, R., Yu, S., Jin, H."Sulforaphane protects against 6-hydroxydopamine-induced cytotoxicity by increasing expression of heme oxygenase-1 in a PI3K/Akt-dependent manner". Molecular Medicine Reports 5, no. 3 (2012): 847-851. https://doi.org/10.3892/mmr.2011.731